| 不同生物反应器中基因工程菌生物强化处理阿特拉津研究 |
| 摘要点击 2867 全文点击 1512 投稿时间：2010-04-01 修订日期：2010-05-12 |
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| 中文关键词 基因工程菌 atzA基因 生物强化 荧光原位杂交 阿特拉津 |
| 英文关键词 genetically engineered microorganism atzA gene bioaugmentation fluorescence in situ hybridization (FISH) atrazine |
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| 中文摘要 |
| 在膜-生物反应器（MBR）和复合生物反应器中，考察基因工程菌生物强化处理阿特拉津去除效果，并对基因工程菌浓度和降解基因atzA基因丰度变化进行检测.结果表明，阿特拉津对COD和氨氮的生物去除活性具有一定的抑制作用；基因工程菌生物强化后，COD及氨氮的去除效率得到恢复.MBR对COD和氨氮的去除效果优于复合生物反应器.基因工程菌生物强化显著提高阿特拉津的生物去除效率，MBR和复合生物反应器阿特拉津去除率均逐渐提高，运行后期平均去除率分别达到38.94%和29.36%.接种基因工程菌后，反应器中基因工程菌细胞浓度快速下降，而后趋于稳定.MBR、复合生物反应器悬浮污泥和附着生物膜稳定细胞浓度分别为5×103、 1.1×103和0.4×103 CFU/mL.采用FISH技术对MBR和复合生物反应器中降解基因atzA基因进行检测，结果表明，atzA基因平均相对丰度先减小而后增加.MBR污泥atzA基因平均相对丰度最大；附着生物膜atzA基因平均相对丰度高于悬浮污泥.atzA基因水平迁移可能是维持较高基因丰度的重要原因. |
| 英文摘要 |
| Removal of atrazine was investigated when genetically engineered microorganism (GEM) was inoculated into membrane bioreactor (MBR) and hybrid bioreactor for bioaugmentation.The performances of atrazine removal in two bioreactors were explored.The variations of GEM density and atzA gene abundance in two bioreactors were also determined.The results indicated that removal activities of COD and ammonia nitrogen were inhibited a little by atrazine and recovered after bioaugmentation by inoculated GEM.The better removal performance of COD and ammonia nitrogen was obtained in MBR.The biological removal efficiency of atrazine was improved significantly when bioaugmented treatment by GEM was applied.The atrazine removal increased gradually and the average removal rates reached up to 38.94% in MBR and 29.36% in hybrid bioreactor in the later running period.After inoculated,GEM densities in two bioreactors decreased rapidly and then tended to be constant.The stable GEM densities in MBR,suspended sludge and adherent biofilm of hybrid bioreactor were 5×103 CFU/mL,1.1×103 CFU/mL and 0.4×103 CFU/mL,respectively.Fluorescence in situ hybridization (FISH) was used to detect atzA gene in two bioreactors and the result indicated that the average relative abundances of atzA gene decreased initially and increased subsequently.The largest average relative abundance of atzA gene was obtained in MBR.The average relative abundance of atzA gene in adherent biofilm is larger than that in suspended sludge in the hybrid bioreactor.The horizontal transfer of atzA gene was the possible important reason responsible for high gene abundance. |
