| 不同底物驯化氧化亚铁硫杆菌的差异及对煤炭生物脱硫效率的影响 |
| 摘要点击 1187 全文点击 1356 投稿时间：2010-03-30 修订日期：2010-06-17 |
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| 中文关键词 嗜酸性氧化亚铁硫杆菌 FeS2 驯化 胞外多聚物 煤炭脱硫 |
| 英文关键词 Acidithiobacillus ferrooxidans pyrite acclimation extracellular polymeric substance coal desulfurization |
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| 中文摘要 |
| 分别以FeS2和FeSO4为底物培养驯化嗜酸性氧化亚铁硫杆菌（Acidithiobacillus ferrooxidans）LX5，结果发现以FeS2为底物驯化A. ferrooxidans LX5，在培养20 d时，培养液中LX5的菌体数量达3.0×107个/mL，对FeS2中亚铁及还原性硫的氧化能力均较强.A. ferrooxidans LX5在以FeSO4为底物的培养液中生长，培养48 h溶液中Fe2+全部氧化成Fe3+，菌体数量达到1.0×108个/mL.以FeS2为底物驯化培养A. ferrooxidans LX5产生的胞外多聚物的量（以DOC计）达到2395 μg/(1010cells)，是同等条件下经FeSO4培养后产生EPS量的3倍.分别将经过FeS2和FeSO4驯化培养的A. ferrooxidans LX5菌液作接种物，进行高硫煤生物浸出脱硫实验，结果表明，生物浸出脱硫13 d，接种FeS2驯化的A. ferrooxidans LX5处理，脱硫率达72.4%，而接种FeSO4培养的A. ferrooxidans LX5处理，反应13 d煤炭脱硫率仅为47.2%，反应20 d煤炭脱硫率达65.8%.因此，以FeS2为底物驯化A. ferrooxidans LX5可以提高煤炭的生物脱硫效率并缩短脱硫时间. |
| 英文摘要 |
| In this study, Acidithiobacillus ferrooxidans LX5 was cultured in 9K medium with pyrite and ferrous sulfate as the substrates. Results showed that the number of A. ferrooxidans LX5 acclimated by pyrite for 20 d was 3.0×107 cell/mL in the liquid, and the oxidation activity to pyrite was stronger. A. ferrooxidans LX5 cultured for 48 h was 1.0×108 cell/mL in medium with FeSO4. Extracellular polymeric substance(EPS) produced from A. ferrooxidans LX5 acclimated by pyrite was 2395 μg/(1010 cells) three times as much as that cultured with FeSO4. A high-sulfur coal desulfurization experiment were carried out with A. ferrooxidans LX5 domesticated respectively in medium with ferrous sulfate and pyrite. The results showed that the coal desulfurization rate was up to 72.4% after bioleaching for 13 d by A. ferrooxidans LX5 acclimated by pyrite, at the same time, the desulfurization rate was only 47.2% with A. ferrooxidans LX5 cultured with ferrous sulfate and reached 65.8% in 20 d. Therefore, the desulfurization efficiency can be improved of coal and shorten the desulfurization time when A. ferrooxidans LX5 acclimated with pyrite as the substrate. |
