| 1株咔唑降解菌的分离、鉴定及其降解特性研究 |
| 摘要点击 1254 全文点击 844 投稿时间：2009-11-09 修订日期：2010-01-15 |
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| 中文关键词 咔唑 生物降解 杂环化合物 16S rRNA 土壤 |
| 英文关键词 carbazole heterocyclic compounds biodegradation 16S rRNA soil |
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| 中文摘要 |
| 利用微生物分离技术，从土壤中分离到1株咔唑降解菌KH-6.菌株KH-6能够以咔唑为唯一的碳源和能源良好地生长，同时还可以利用3-甲基咔唑、 4-羟基咔唑和2,2′-二羟基联苯生长.经过对其形态特征、生理生化、以及16S rDNA序列分析，初步鉴定为黄杆菌属细菌.该菌株对咔唑的降解性是通过在液体培养基内菌体的增加及底物的减少来证实的，其利用咔唑生长的最适温度和pH分别为30℃和7.5.静止细胞反应试验表明，菌株KH-6除了咔唑以外还可降解其它杂环化合物.咔唑污染土壤中的生物降解实验表明，在灭菌土壤中90%的咔唑被降解，菌株KH-6对土壤中的咔唑有很好的生物降解效果. |
| 英文摘要 |
| A bacterial strain was isolated from soil samples using plate screening techniques. Results indicated this isolated were able to use carbazole as sole source of carbon and energy, simultaneously, including N-Methylcarbazole, 4-Hydroxycarbazole and 2,2′-Biphenol. It was identified as Flavobacteriumsp. according to its morphology, and biochemical properties, and 16S rDNA sequence analysis. Utilization of carbazole by the isolates was confirmed by the increase in bacterial biomass and the decrease in substrate concentration in liquid cultures. The optimal pH and temperature for cell growth and carbazole degradation were 7.5 and 30℃, respectively. Resting cells grown in Luria broth also showed activity for decomposing other heterocyclic compounds. In addition, biodegradation of carbazole was carried out with carbazole degrading strain KH-6. The results indicated that 90% of the carbazole could be degraded in the sterilized soil. And strain KH-6 could enhance the degradations of carbazole significantly. |
