| 热纤维梭菌不同菌株的纤维素降解和酒精生产能力 |
| 摘要点击 1370 全文点击 1247 投稿时间：2009-10-17 修订日期：2009-12-08 |
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| 中文关键词 纤维素酒精 热纤维梭菌 嗜热厌氧乙醇菌 纤维素降解 酒精生产能力 |
| 英文关键词 cellulosic ethanol Clostridium Thermoanaerobacter cellulose degradation ethanol production |
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| 中文摘要 |
| 以高温厌氧细菌热纤维梭菌 (Clostridium thermocellumLQRI和VPI菌株) 和嗜热厌氧乙醇菌 (Thermoanaerobacter ethanolicusX514和39E菌株) 为对象，以不同浓度纤维素为底物，分析了热纤维梭菌不同菌株的纤维素降解和酒精生产能力.结果表明，在热纤维梭菌纯培养体系中，LQRI对纤维素的降解能力明显高于VPI，前者约为后者的1.2倍，但两者的酒精生产能力没有显著差异，LQRI在1%纤维素条件下酒精终浓度约为12 mmol/L，VPI约为10 mmol/L.随着底物纤维素浓度的增加 (1%、 2%、 5%)，两者对纤维素降解和酒精生产能力均呈明显下降趋势.在热纤维梭菌和嗜热厌氧乙醇菌混合培养体系中，LQRI对纤维素的降解能力明显高于VPI，前者约为后者的1.28～1.58倍.随着底物浓度的增加，两者的纤维素降解率均逐渐下降，但热纤维梭菌对纤维素降解的绝对数值还受到其它混合培养菌种的影响.此外，LQRI+Thermoanaerobacter混合培养体系的酒精生产能力明显高于VPI+Thermoanaerobacter，前者约为后者的1.27～1.77倍.随着底物浓度的增加，混合培养体系酒精生产能力没有明显下降趋势. |
| 英文摘要 |
| Cellulose degradation and ethanol production of two types of cellulosic materials with different concentration were evaluated in batch system of mono-cultures of cellulolytic ethanol producing strains (Clostridium thermocellumstrain LQRI and Clostridium thermocellumstrain VPI), and co-cultures of LQRI or VPI in combination with one of the non-cellulolytic ethanol producing strains (Thermoanaerobacter ethanolicus strains X514 or Thermoanaerobacter ethanolicus39E). Results demonstrated that higher cellulose degradation abilities about 1.2 times were detected in LQRI mono-culture than in VPI mono-culture, while no significant difference of ethanol yields was found between the two mono-cultures. Abilities of cellulose degradation and ethanol production decreased significantly with the increasing of substrate cellulose concentration (1%, 2%, 5%). In the co-culture system, cellulose degradation abilities of LQRI were also significantly higher than VPI, the former is 1.28-1.58 times of the latter. Cellulose degradation rate of LQRI + Thermoanaerobacterand VPI+ Thermoanaerobacterdecreased gradually with the increasing of substrate cellulose concentration, while the absolute value of cellulose degradation was also affected by the partner Thermoanaerobacter strain. Additionally, the ethanol yields in the co-cultures of LQRI+Thermoanaerobacterwere significantly higher than that in the co-cultures of VPI+Thermoanaerobacter with same Thermoanaerobacterpartner, the former is 1.27-1.77 times of the latter. However, ethanol yields in the co-cultures have not significantly declined with the increasing of substrate cellulose concentration. |
