| A strain of photosynthetic bacteria named PSB-1D with degradation of o-chlorophenol (2-CP) was isolated and screened from the shallow substrate sludge in downstream side of the sewage outfall of an insecticide factory.The PSB-1D is identified preliminarily as Rhodopseudomonas sp.according to its colony and cell morphological properties,physiological biochemical characteristics and absorption spectrum analysis of living cells.The experiments results of relationship between PSB-1D growth and o-chlorophenol degradation showed that the degradation rate of o-chlorophenol was up to 57.26% after 7 days cultural time.The main environmental factors including way of illumination and oxygen,initial pH,cultural temperature,illumination intensity had distinctly influenced on the o-chlorophenol degradation with PSB-1D.The results showed that the optimum conditions were as following:an anaerobic light,pH 7.0,temperature 30℃,illumination intensity 4000 lx,initial o-chlorophenol concentration 50 mg/L.Under that cultural condition,the degradation rate of o-chlorophenol could reach to 62.08%.The degradation kinetic data fitted the Andrews model well.In addition,the biodegradation process of o-chlorophenol can be well described by enzymatic reaction of high concentration inhibition,with the maximum substrate utilization rate 0.309 d-1,Michaelis-Menten constant 2.733 mg/L,inhibitory constant 230.15 mg/L respectively. |
