首页  |  本刊简介  |  编委会  |  投稿须知  |  订阅与联系  |  微信  |  出版道德声明  |  Ei收录本刊数据  |  封面
T-RFLP方法分析城市污水处理厂中细菌群落的动态变化
摘要点击 2883  全文点击 1913  投稿时间:2009-07-06  修订日期:2009-08-31
查看HTML全文 查看全文  查看/发表评论  下载PDF阅读器
中文关键词  细菌  群落动态变化  T-RFLP  移动窗口分析  洛伦兹曲线  城市污水处理厂
英文关键词  bacteria  community dynamics  terminal restriction fragment length polymorphism (T-RFLP)  moving window analysis  Lorenz curve  wastewater treatment plant
作者单位
王晓慧 清华大学环境科学与工程系北京100084 
文湘华 清华大学环境科学与工程系北京100084 
丁鵾 清华大学环境科学与工程系北京100084 
张辉 北京排水集团北京100044 
周军 北京排水集团北京100044 
中文摘要
      采用基于16S rRNA基因的末端限制性片段长度多态性技术(T-RFLP),分析了功能稳定的2个城市污水处理系统中(系统A及系统B),细菌群落结构在105 d中的动态变化.在研究中,系统A和系统B功能稳定,BOD出水浓度均在8 mg/L左右,总氮(TN)出水浓度分别在25 mg/L和23 mg/L之下.T-RFLP图谱分析表明,系统A中,115、 118、 123、 471、 482和835 bp等T-RFs的相对丰度变化显著,系统B中,123、 471、 750和835 bp等T-RFs的相对丰度变化显著.移动窗口分析表明,系统A中细菌群落的平均变化率Δt(15 d) 为21.5%±5.7%,系统B的平均变化率Δt(15 d) 为22.6%±5.0%.洛伦兹曲线分析表明,系统A中20%的微生物的累积相对丰度为43%~50%;系统B中20%的微生物的累积相对丰度为47%~52%. 结果表明,在城市污水处理厂中,稳定的功能并没有藕联着稳定的细菌群落结构.
英文摘要
      Bacterial communities in two full-scale wastewater treatment systems (system A and system B) with functional stability were monitored over 105 days using terminal restriction fragment length polymorphism (T-RFLP) based on 16S rRNA genes. During the study period, system A and system B were functionally stable. The effluent BOD concentration of two systems was always around 8 mg/L, and at the same time the effluent TN concentrations of two systems were always below 25 mg/L and 23 mg/L respectively. However, the T-RFLP profiles showed that for system A, the relative abundance of T-RF of 115, 118, 123, 471, 482 and 835 bp changed significantly. For system B, the T-RF of 123, 471, 750 and 835 bp changed significantly. The moving window analysis showed the average community change rates (every 15 days) of system A and system B were 21.5%±5.7% and 22.6%±5.0% respectively.Based on the Lorenz curves, it was observed for system A that 20% of the species corresponded with 43% to 50% of the cumulative relative abundance. For system B, 20% of the species corresponded with 47% to 52% of the cumulative relative abundance. In conclusion, the results of this study clearly revealed that, in the full-scale wastewater treatment systems, the functional stability did not correlate to the stable bacterial communities.

您是第52872440位访客
主办单位:中国科学院生态环境研究中心 单位地址:北京市海淀区双清路18号
电话:010-62941102 邮编:100085 E-mail: hjkx@rcees.ac.cn
本系统由北京勤云科技发展有限公司设计  京ICP备05002858号-2