| 1株BTEX降解新菌株的分离鉴定及其降解特性研究 |
| 摘要点击 1996 全文点击 1786 投稿时间：2009-08-10 修订日期：2009-10-13 |
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| 中文关键词 苯系物 降解 分枝杆菌属 比耗氧速率 动力学 |
| 英文关键词 benzene,toluene,ethyl benzene,and o-xylene(BTEX) biodegradation Mycobacterium sp. specific oxygen utilization rates kinetics |
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| 中文摘要 |
| 取自炼油污水处理厂曝气池的活性污泥经过苯系物定向驯化后，选育到1株能同时高效降解苯、甲苯、乙苯和邻二甲苯（BTEX）的菌株byf-4，基于形态特征、生理生化、16S rDNA序列系统学分析和Biolog鉴定，可确定该菌株为染料分枝杆菌Mycobacterium cosmeticum，其为新发现的1株具有降解BTEX性能的菌株.该菌株最佳生长温度和pH分别为30℃和7.0，其对4种苯系物的降解优先顺序为苯、甲苯、乙苯、邻二甲苯；菌株降解苯、甲苯、乙苯和邻二甲苯的比耗氧速率分别为165.3、170.5、49.3和57.4 mg·(min·mg)-1；菌株降解BTEX的过程遵循Haldane动力学模型，对苯、甲苯、乙苯和邻二甲苯的最大比降解速率分别为0.518、0.491、0.443和0.422 h-1，菌株最大比生长速率分别为0.352、0.278、0.172和0.136 h-1. |
| 英文摘要 |
| A bacterial strain,able to efficiently degrade benzene,toluene,ethyl benzene,and o-xylene(BTEX) compounds,was isolated by acclimating and enriching the activated sludge from the aeration tank in refinery wastewater treatment plant using BTEX as the sole carbon source.Based on the morphological characteristics,physiological and biochemical characteristics,sequence analysis of 16S rDNA,and Biolog identification system,the isolate was identified as Mycobacterium cosmeticum which was a newly discovered species able to degrade BTEX.The optimal conditions for the growth of the strain were at 30℃ and pH 7.0. The order of BTEX degradation by this isolate is benzene,toluene,ethyl benzene,and o-xylene.The specific oxygen utilization rates (SOUR) of the strain degrading benzene,toluene,ethyl benzene,and o-xylene were 165.3,170.5,49.3 and 57.4 mg·(min·mg)-1,respectively.The degrading process of the strain followed the Haldane kinetic model.The maximum specific degradation rate degrading benzene,toluene,ethyl benzene,and o-xylene were 0.518,0.491,0.443 and 0.422 h-1,respectively.Accordingly,the maximum specific growth rate 0.352,0.278,0.172 and 0.136 h-1,respectively. |
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