| 萘胁迫对水稻基因组DNA甲基化模式及水平的影响 |
| 摘要点击 2080 全文点击 2107 投稿时间：2008-12-25 修订日期：2009-09-11 |
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| 中文关键词 水稻 日本腈 松前 萘 DNA甲基化 甲基化敏感扩增多态性 |
| 英文关键词 rice Nipponbare Mastumae naphthalene DNA methylation methylation-sensitive amplification polymorphism(MSAP) |
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| 中文摘要 |
| 运用MSAP技术测定水稻(Oryza sativa L.)纯系品种‘日本腈’和‘松前’经萘染毒胁迫后，不同生长时期叶片基因组DNA甲基化变异的情况.结果表明，水稻经萘胁迫后存在基于DNA甲基化水平和模式改变的表观遗传变异；5-甲基胞嘧啶百分含量的变化无统一趋势或规律；全部检测到的1051个位点中，日本腈有16.56%发生了变异，相对于松前的12.08%具有显著差异，一定程度上说明抗萘胁迫能力与基因型有关：松前强于日本腈；不同基因型及不同生长时期DNA甲基化模式的变异存在明显差异，表现为以去甲基化为主（0.48%～10.41%），超甲基化较少（0.10%～1.92%），由此推测DNA去甲基化可能是植物抗萘胁迫机制的一部分. |
| 英文摘要 |
| In this study,two elite rice inbred lines,Nipponbare and Mastumae were selected and irrigated with naphthalene solution with different concentrations.Epigenetic instabilities in the blade of the plants in tillering and heading stage were assessed by MSAP marks.The results showed that naphthalene exposure induced epigenetic variations in all the samples based on different levels and patterns of DNA methylation.The changes of the percentage of 5-methylcytosine have no regulation.In total 1051 sites tested,the variation of 16.56 percentage of Nipponbare has significant difference compared with the variation of 12.08 percentage of Mastumae,which showed that the capability to resist naphthalene pollution was related to genotype and Mastumae was stronger than Nipponbare in the capability to resist naphthalene pollution.The variations of DNA methylation in different genotypes and different growth periods have significant differences.In general,the frequencies of demethylation (0.48%-10.41%）were higher than hypermethylation (0.10%-1.92%).We concluded that DNA demethylation might be one part of in plants mechanism to resist naphthalene pollution in plants. |
