| 铜对颤蚓的快速灭活机制及其在水厂应用分析 |
| 摘要点击 2446 全文点击 2268 投稿时间：2009-03-19 修订日期：2009-08-03 |
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| 中文关键词 颤蚓 铜 灭活 羟基自由基(·OH) 过氧化氢酶(CAT) 丙二醛(MDA) |
| 英文关键词 T. tubifex copper inactivation hydroxyl radical (·OH) catalase (CAT) malondialderhyde (MDA) |
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| 中文摘要 |
| 采用对苯二甲酸(terephthalic acid, TA)作为羟基自由基(hydroxyl radical, ·OH)捕捉剂, 利用荧光分光光度法对铜暴露30 min后颤蚓体内·OH浓度进行测定, 并结合灭活率、过氧化氢酶(catalase, CAT)和丙二醛(malondialderhyde, MDA)等指标测试, 研究了铜对颤蚓的灭活效果及毒性作用机制. 结果表明, 铜对颤蚓具有高效快速灭活作用, 1 mg·L-1暴露条件下灭活率即可达100%;其主要灭活机制之一是诱导颤蚓体内 |
| 英文摘要 |
| The fluctuation of ·OH concentration in Tubifex tubifex exposed to copper was investigated by Fluorospectrophotometry using terephthalic acid (TA) as ·OH trapping agent. Catalase (CAT) activity in T. tubifex was quantified after 30 minutes contact with copper. Inactivation effect and lipid peroxidation produce malondialderhyde (MDA) of T. tubifex were also studied. Results showed that copper had significant inactivation effect on T. tubifex. 100% inactivation efficiency was obtained when copper levels was 1 mg·L-1. One of the inactivation mechanisms is inducing ·OH increase in T. tubifex, which results in oxidative stress and lipid peroxidation. The increment of ·OH concentration was 69.2% when copper lever was 0.05 mg·L-1. The significant decrease of CAT activity showed copper could inhibit CAT activity to increase its oxidative stress to T. tubifex. Between 0.05-0.25 mg·L-1, copper induced significant MDA increase, which meant lipid peroxidation. The changes of inactivation efficiency, ·OH, CAT and MDA showed that the inactivation effect of copper on T. tubifex did not result only from oxidative stress and lipid peroxidation induced by ·OH itself. Feasibility analysis of using copper to inactivate T.tubifex for water plant showed when sand filter was immersed by 1 mg·L-1 copper for 30 min and then began backwashing, residual copper concentration was only 4.3 μg·L -1. |
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