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UV-C辐照抑制铜绿微囊藻生长的动态实验研究
摘要点击 1524  全文点击 1363  投稿时间:2009-04-02  修订日期:2009-06-10
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中文关键词  铜绿微囊藻  紫外线反应器  UV-C辐照  藻细胞生长抑制  动态实验
英文关键词  Microcystis aeruginosa  UV reactor  UV-C irradiation  growth suppression on algae cells  dynamic experiment
作者单位
袁侃 清华大学深圳研究生院环境工程与管理研究中心深圳518055 
毛献忠  
陶益  
张锡辉  
中文摘要
      以我国典型淡水水华藻种铜绿微囊藻(Microcystis aeruginosa 905)为研究对象,采用推流式低压紫外灯反应器研究在动态条件下Ultraviolet-C(UV-C)辐照对蓝藻生长抑制作用的效果.对数培养期的蓝藻液流经反应器进行UV-C辐照处理,其后正常培养,在培养期2 h、1 d、3 d、5 d、7 d、9 d分别取样,通过研究级倒置荧光显微镜在荧光视野下进行细胞计数.结果表明,辐照处理未引起铜绿微囊藻细胞的大量破裂死亡,36~115 mW·s·cm -2
英文摘要
      A plug-flow UV-C reactor equipped with low pressure UV lamp was utilized to study the suppression effect after UV-C irradiation under the dynamic conditions on Microcystis aeruginosa, a typical cyanobacterium in algae blooms in China. The culture fluid of Microcystis aeruginosa was exposed to UV-C irradiation when pumped through the reactor. After that, the fluid was incubated under the normal culture condition, and sampled at 2 h, 1 d, 3 d, 5 d, 7 d, 9 d for determination of cell density using the inverted system fluorescence microscope. The experiments showed that, UV-C irradiation did not cause severe cell lysis, and UV-C irradiation at dose ranged from 36 to 115 mW·s·cm-2, and 31 to 50 mW·s·cm-2 could suppress Microcystis aeruginosa biomass growth for the 2.6×105-2.7×105 cells·mL-1 and 9.0×105-1.15×106 cells·mL-1 fluid in 9 days, respectively.

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