| 苜蓿根瘤菌对多氯联苯降解转化特性研究 |
| 摘要点击 1517 全文点击 1711 投稿时间：2008-09-05 修订日期：2009-06-15 |
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| 中文关键词 苜蓿根瘤菌 多氯联苯 微生物降解 生物转化 |
| 英文关键词 Rhizobium meliloti polychlorinated biphenyls microbial degradation biotransformation |
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| 中文摘要 |
| 采用溶液摇瓶实验研究了苜蓿根瘤菌(Rhizobium meliloti)对三氯代联苯(2,4,4′-trichlorobiphenyl)单体以及18种多氯联苯(polychlorinated biphenyls，PCBs)混合物的降解转化能力.结果表明，接入苜蓿根瘤菌转化7d后，随着溶液中底物2,4,4′-TCB浓度的增加，该菌株对其的降解能力也逐渐提高，在1.0、5.0、10.0、25.0、50.0 mg·L-1浓度条件下该菌对2,4,4′-TCB的降解率分别为34.0%、48.3%、69.7%、96.0%、98.5%，并且通过气相质谱分析，发现溶液中出现了一些新的代谢产物.同时，苜蓿根瘤菌对不同浓度下18种PCBs同系物混合物的降解能力，随着底物浓度的升高呈现出从低到高，然后降低，并趋于平衡的趋势，其最高降解率可达54.7%，并存在高氯代PCBs向低氯代PCBs的转化过程. |
| 英文摘要 |
| The capability of Rhizobium meliloti on degrading 2,4,4′-trichlorobiphenyl and 18 kinds of polychlorinated biphenyl congener mixtures was studied by shaking flask experiment．The results showed that the degradation capability of Rhizobium meliloti to 2,4,4′-trichlorobiphenyl increased gradually with the increasing concentration of 2,4,4′-trichlorobiphenyl. After 7 days, the degradation rates of 2,4,4′-trichlorobiphenyl under 1.0, 5.0 10.0, 25.0, 50.0 mg·L-1 concentration of 2,4,4′-trichlorobiphrnyl were 34.0%，48.3%，69.7%，96.0%，98.5% respectively. Meanwhile some new intermediate products were found in the solution culture. The capability of Rhizobium meliloti on degrading 18 kinds of polychlorinated biphenyls congener mixtures appeared in a trend from low to high, then decreased to a certain balance with the increasing concentration of the mixture. The highest degradation rate was 54.7%, and moreover, a transformation process from higher chlorinated PCBs congeners to lower chlorinated PCBs congeners emerged. |
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