| 基于Biolog解析添加酶液对堆肥化过程中微生物群落代谢的影响 |
| 摘要点击 1423 全文点击 2882 投稿时间：2008-11-16 修订日期：2009-03-16 |
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| 中文关键词 堆肥 微生物群落代谢 Biolog方法 聚类分析 PCA分析 |
| 英文关键词 compost microbial community metabolism Biolog method cluster analysis PCA |
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| 中文摘要 |
| 以Biolog方法为主要监测手段，结合聚类分析以及PCA分析方法，考察了添加酶液对农业废物堆肥中木质纤维素降解及微生物群落代谢能力的影响.结果表明，添加酶液使得堆料中有机质的降解率提高了4.90%；微生物群落代谢聚类结果表明，添加酶液可以改善堆肥微生物对中间代谢产物类碳源的代谢能力.通过PCA发现，添加酶液提高了堆体中微生物对双亲化合物、聚合物、氨基酸和氨基化合物等碳源的代谢能力，由此可导致有机质被更高效降解；此外，堆肥各时期群落代谢聚类结果表明，酶液对堆肥进程的影响主要表现在一次发酵的第6 d和二次发酵的第30 d，从而有效地加速了堆肥进程. |
| 英文摘要 |
| The effects of enzymes on organic material degradation and microbial communities metabolic profiles during composting process were studied using Biolog method, and together with cluster analysis and PCA. The results showed that, adding the enzyme solution in the composting could increase the degradation rate of organic material by 4.90%. The microbial community metabolic results of cluster analysis showed that when the enzyme solution was added into the compost, the carbon metabolic capability of intermediate metabolite was improved. The results of PCA indicated that when the enzyme solution was added, microbial communities enhanced the metabolic capability of miscellaneous, polymers, amino acids and amides carbon substrates, which results in the efficient degradation of organic substance. In addition, cluster analysis of each composting phase showed that the effects of the enzymes solution on microbial community metabolism were mainly observed on 6 d and 30 d, which promoted the composting process. |
