| 太湖水体中碱性磷酸酶的空间分布及生态意义 |
| 摘要点击 2586 全文点击 1485 投稿时间：2008-11-20 修订日期：2009-02-16 |
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| 中文关键词 太湖 碱性磷酸酶活性(APA) 最大反应速率Vmax 米氏常数Km 空间分布特征 |
| 英文关键词 Taihu Lake alkaline phosphatase activity(APA) Vmax Km spatial distribution characteristics |
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| 中文摘要 |
| 在对太湖生态湖区进行分区的基础上，监测各湖区水体碱性磷酸酶活性、动力学参数以及常规水环境化学指标，探讨水体碱性磷酸酶活性的空间分布特征及其环境影响因素.结果表明，太湖水体碱性磷酸酶活性(APA)、最大反应速率(Vmax)值及碱性磷酸酶反应米氏常数(Km)值分布均呈空间异质性；APA与Vmax值具有相似性的空间分布规律，即西岸河口区水体中APA值与Vmax值最大，分别为(9.43±5.30) nmol·(L·min)-1和(13.70±7.42) nmol·(L·min)-1，在其他湖区依次为湖心区＞草型湖区＞梅梁湾区＞竺山湖区＞贡湖区；草型湖区Km值(20.50±11.30) μmol·L-1＞贡湖区＞竺山湖区＞梅梁湾区＞湖心区＞西岸河口区(9.17±3.46) μmol·L-1.太湖水体中Vmax与pH、总磷(TP)、叶绿素a(Chla)之间均存在显著的线性正相关，其相关系数分别为rpH=0.651 2**(p<0.01)、rTP=0.488 5**(p<0.01)、rChla=0.765 6**(p<0.01)，但与水温、溶解性总磷(DTP)、正磷酸盐(PO3-4-P)无显著的相关性；Km值与TP浓度间呈显著的线性负相关性，相关系数为rTP=-0.383 4*(p=0.048)，与水温、pH、DTP、PO3-4-P以及Chla浓度的相关性不显著. |
| 英文摘要 |
| Based on different ecological zone of Taihu Lake, alkaline phosphatase activity (APA), the kinetic parameters and the chemical parameters in water column from different zone of Taihu Lake were monitored, and the spatial distribution characteristics and the effects of environmental factors on the values of APA, Vmax and Km were studied. The results showed that the values of APA, Vmax and Km in water column from Taihu Lake had a spatial heterogeneous distribution. The spatial distribution characteristic of APA values was the same as that of Vmax ones in water from different zones of Taihu Lake, namely, the maximal values of APA (9.43±5.30) nmol·(L·min)-1 and Vmax (13.70±7.42) nmol·(L·min)-1 occurred in water from estuary zone in western bank of Taihu Lake. The value distribution of APA and Vmax in other zone of Taihu Lake followed as: the central zone of Taihu Lake>the grass type zone of Taihu Lake>the Meiliang Bay zone of Taihu Lake>the Zhushan Bay zone of Taihu Lake>the Gonghu Bay zone of Taihu Lake. The value of Km from the grass type zone of Taihu Lake was the highest (20.50±11.30) μmol·L-1, and the one from estuary zone in western bank of Taihu Lake was the lowest (9.17±3.46) μmol·L-1. The value of kinetic parameter Vmax was significantly positively correlated with the values of pH, total phosphorus (TP) and the chlorophyll a (Chla), with rpH=0.651 2** (p<0.01), rTP=0.488 5** (p<0.01) and rChla=0.765 6** (p<0.01), respectively. However, the effects of hydro-temperature, dissolved total phosphorus (DTP) and orthophosphorus (PO3-4-P) on Vmax values were negligible. There was no significant influence of the hydro-temperature, pH, DTP, PO3-4-P and Chla concentrations on the Km values, nevertheless significant negative relationship between the Km value and TP content was found with r=-0.383 4* (p=0.048). |
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