| In order to investigate the combined effects of antibiotics on anaerobic digestion of piggery wastewater, batch tests based on orthogonal test were adopted. Amoxicillin, aureomycin, sulfadimethoxine, florfenicol which are the most common antibiotics used in the prevention and treatment of pig diseases were chosen in the experiment. The results showed that the methane production rate was affected most by the combination of 130 mg/L of florfenicol, 210 mg/L of amoxicillin, 10 mg/L doxycycline and 210 mg/L sulfadimethoxine and the inhibitory effect on maximum methane production rate amounted to 87.8%. No inhibitory effect but stimulus was observed on anaerobic digestion in the presence of florfenicol, amoxicillin, aureomycin, sulfadimethoxine with 10 mg/L. The significance of the inhibitory effect of the selected antibiotics was in an order of aureomycin, amoxicillin, florfenicol and sulfadimethoxine (p>0.005). Different kinds of first-order interactions between either two antibiotics were concluded from the experiment. Except that florfenicol was in a additivity relationship with amoxicillin and sulfadimethoxine the interaction in other antibitotics was antagonistic. |
