| 对氨基苯磺酸生物降解动力学及降解机制研究 |
| 摘要点击 2278 全文点击 1192 投稿时间：2008-09-05 修订日期：2008-11-05 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 对氨基苯磺酸 生物降解 降解动力学 代谢途径 |
| 英文关键词 4-aminobenzenesulphonate(4-ABS) biodegradation kinetics pathway |
|
| 中文摘要 |
| 对菌株Pannonibactersp.W1在好氧条件下降解对氨基苯磺酸的动力学进行了研究，发现4-ABS初始浓度为中低浓度(50～1 000 mg/L)时， 14 h内几乎可以完全降解，符合一级降解动力学特征；初始浓度为高浓度(1 200～2 500 mg/L)时，32 h内4-ABS的降解率可达90%以上，且在降解初期符合零级降解动力学特征，而降解后期符合一级降解动力学特征.利用Haldane抑制模型能够很好地拟合不同4-ABS初始浓度下测得的比降解速率，得到模型参数分别为：最大比降解速率μmax=227.977 mg/(g·h)，饱和常数Ks=84.306 mg/L，抑制常数Ki=1270.675 mg/L.通过4-ABS降解过程中的紫外扫描和HPLC检测分析，以及菌株W1对不同苯系物的降解能力，说明4-ABS降解过程中几乎没有芳香类中间产物的积累，并初步推测了菌株W1降解4-ABS的代谢途径. |
| 英文摘要 |
| Kinetics of 4-aminobenzenesulphonate(4-ABS) degradation by Pannonibactersp.W1 under aerobic condition was studied. At medium and low initial concentration (50-1 000 mg/L)，the 4-ABS could be degraded almost completely in 14 h，and the degradation reaction conformed to one order kinetics. At high initial concentration (1 200-2 500 mg/L)，up to 90% degradation rate could be achieved in 32 h，and it conformed to zero order kinetics in earlier period of degradation and one order kinetics in later period of degradation. Haldane inhibition model was used to fit the special degradation rate at different initial concentration，and the parameters μmax，Ks and Ki were determined to be 227.977 mg/(g·h)， 84.306 mg/L and 1270.675 mg/L，respectively. UV spectrum and HPLC detection indicated that no other aromatic intermediates were accumulated during the 4-ABS degradation. The degradation of other substituted benzenes by strain W1 were detected，and the metabolic pathway of 4-ABS degradation was proposed. |
