| 1株产电假单胞菌（Pseudomonas sp.）RE7的分离及特性研究 |
| 摘要点击 2352 全文点击 993 投稿时间：2008-08-26 修订日期：2008-11-19 |
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| 中文关键词 MFC 假单胞菌 产电特性 |
| 英文关键词 microbial fuel cells (MFCs) Pseudomonas electricity production |
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| 中文摘要 |
| 微生物燃料电池(microbial fuel cell，MFC)阳极微生物的种类和作用机制对MFC的产电性能有着重要的影响.从已稳定运行1 a的MFC的阳极室分离得到1株电化学活性革兰氏阴性细菌——菌株RE7，其16S rRNA基因序列与Pseudomonas aeruginosa strain CMG 587有99%同源性，属于假单胞菌属（Pseudomonas sp.）.利用菌株RE7构建的MFC的稳定产电和循环伏安曲线测定结果都表明，菌株RE7具有较强的电化学活性，利用菌株RE7构建的MFC的最大输出电压为352 mV，相应的最大面积功率密度为69.2 mW/m2，体积最大功率密度为6.2 W/m3.由不同稀释比例的MFC排出液的产电效果比较可知，菌株RE7极有可能是通过自身分泌的氧化还原类物质进行电子传递. |
| 英文摘要 |
| Microbial components of the microbial fuel cells (MFCs)，including species constitution and metabolic mechanism of the anodic microorganisms，are critical to the optimization of electricity generation.An electrogenesis bacterium strain (designated as RE7) was isolated from an MFC that had been running in a fed batch mode for over one year.The isolate was identified as a strain of Pseudomonas aeruginosa based on its physiological，morphological characteristics and 16S rRNA sequence analysis.Direct electron transfer from RE7 to an electrode was examined using cyclic voltammetry and MFC.Results of both methods showed the electrochemical activity of the bacterium without any electrochemical mediator.The P. aeruginosa strain RE7 was inoculated into the anode chamber of a packing-type MFC and the maximal voltage output was 352 mV with 1 500 mg/L glucose as the fuel.Correspondingly，the maximal area and volumetric power densities were 69.2 mW/m2 and 6.2 W/m3，respectively.Bacteria-producing soluble redox mediators，such as phenazine derivatives，are possible mechanism to facilitate the direct electron transfer to the electrode from the bacterial cells. |
