| 碳源对EBPR代谢过程及微生物特性的影响 |
| 摘要点击 2573 全文点击 2110 投稿时间：2008-08-17 修订日期：2008-10-26 |
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| 中文关键词 强化生物除磷 乙酸 丙酸 变性梯度凝胶电泳 扫描电镜 |
| 英文关键词 enhanced biological phosphorus removal acetic acid propionic acid DGGE SEM |
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| 中文摘要 |
| 采用SBR反应器，研究了乙酸和丙酸分别作为唯一进水碳源时对EBPR过程物质转化及代表性微生物变化特性的影响，并对不同碳源可能导致不同的微生物代谢过程进行了讨论.SBR的运行模式为：厌氧2 h，好氧5 h，每天运行3个周期，乙酸和丙酸进水的COD均为300 mg/Ｌ，系统先用乙酸作为碳源运行60 d，随后以丙酸作为碳源运行60 d.结果表明，在采用乙酸作为碳源时，厌氧结束放磷和消耗COD的比值为0.35，生成的PHA中以PHB为主，占92.6%，PHV只占到7.4%，没有PH2MV生成.在采用丙酸作为碳源时，厌氧结束放磷和消耗COD的比值稍低，为0.27，生成的PHA中PHV占35.8%，PHB和PH2MV分别占10.2%和54.0%.2种碳源条件下系统都具有良好的EBPR效能，出水PO3-4-P均在检出限以下.对不同阶段的污泥进行DGGE分析表明，系统中的微生物发生了变化；扫描电镜图片和PHA染色结果分析表明，在乙酸作为碳源时，系统中的PAOs以球菌形式存在，而在丙酸作为碳源时，系统中的PAOs以杆菌形式存在.不同碳源培养出了不同类型的PAOs，两者代谢途径不同，但都具有较好的EBPR效能. |
| 英文摘要 |
| A SBR was used in this study for investigating the influence of carbon source on EBPR metabolism and microorganism communities when feeding with acetate and propionate. The SBR was operated with a cycle time of 8 h and each cycle consisted of 4 min feeding, 2 h anaerobic period, 5 h aerobic period, 35 min setting, 15 min decanting and 6 min waiting. The COD of influent was kept at 300 mg/Ｌ during the experiment. Acetate and propionate were used as the sole carbon source for operation of 60 days, respectively. The phosphorus release/COD consumption in the end of anaerobic phase were 0.35 and 0.27 when acetate and propionate were used as the carbon source, respectively. The PHA composition was different when different carbon source was dosed. PHB accounted for 92.6% in the end of anaerobic phase but the value for PHV was only 7.4% when acetate was selected as the carbon source. No PH2MV was detected during this process. The compositions of PHA were PHB (10.2%), PHV (35.8%) and PH2MV (54.0%) in the end of anaerobic cycle when propionate was used as the sole carbon source. There was variation of microorganism communities during this process for the results of DGGE combined with SEM micrographs and PHA staining. Coccus morphotype PAOs were accumulated in acetate-fed phase and rod morphotype PAOs were accumulated in propionate-fed stage. Different PAOs were accumulated and the metabolic pathways were different when different carbon sources were used, but good EBPR could be achieved during all these conditions. |
