| 醌还原酶 醌类化合物对偶氮染料脱色的作用 |
| 摘要点击 2408 全文点击 1435 投稿时间：2008-09-04 修订日期：2008-10-24 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 基因工程菌 醌还原酶 氧化还原介体 偶氮染料 |
| 英文关键词 genetically engineered bacteria quinone reductase redox mediator azo dyes |
|
| 中文摘要 |
| 外加醌类化合物作介体，利用细菌胞内的醌还原酶考察基因工程菌Escherichia coli YB对偶氮染料的脱色能力，以及甲基氢醌预处理对E. coli JM109和厌氧污泥脱色的影响.结果表明，介体2 羟基 1，4 萘醌（lawsone，LQ）的存在对胞内过量表达醌还原酶AZR的E. coli YB的脱色能力有显著的促进作用，0.2 mmol·L-1 LQ存在下，苋菜红（1 mmol·L-1）在2 h内可脱色75%.LQ存在时E. coli YB对高浓度的染料也有脱色效果，8 h可使苋菜红（5 mmol·L-1）脱色50%左右.与LQ相比，甲萘醌作为介体对脱色的促进效果较差，2.5 mmol·L-1甲萘醌存在下脱色70%需12 h.LQ存在时E. coli YB对苋菜红的重复脱色能力稳定，12 h内能完成4次重复脱色.LQ的存在对于结构较复杂的2种偶氮染料酸性大红GR和活性艳红K 2BP的脱色也有促进作用，在最适LQ浓度下，分别在9 h和30 h脱色70%.甲基氢醌预处理对E. coli JM109和厌氧污泥的脱色能力都有促进作用.预处理后在LQ存在下，E. coli JM109在5 h可脱色苋菜红（1 mmol·L-1）80%左右，而污泥可在11 h脱色75%以上. |
| 英文摘要 |
| Using quinoid redox mediator and bacterial cellular quinone reductase，we investigated the decolorization ability of gene engineered strain Escherichia coli YB and the effects of methylhydroquinone (MHQ) pretreatement on decolorization performance of E. coli JM109 and anaerobic sludge. The results indicate that lawsone is an effective accelerator for azo dye decolorization by E. coli YB overexpressing cellular quinone reductase AZR. In the presence of 0.2 mmol·L-1 lawsone，75% Amaranth (1 mmol·L-1) can be decolorized in 2 h. E. coli YB can also decolorize high concentration of azo dye in the presence of lawsone. Around 50% Amaranth (5 mmol·L-1) is decolorized in 8 h. Compared to lawsone，menadione is a less effective mediator. E. coli YB takes 12 h to reach 70% decolorization in the presence of 2.5 mmol·L-1 menadione. Repeated decolorization studies showed that E. coli YB had stable decolorizing ability in the presence of lawsone. Four rounds of repeated decolorization can be completed in 12 h. Lawsone can also accelerate the decolorization of azo dyes with complex structures such as Acid Scarlet GR and Reactive Brilliant Red K 2BP. With the optimal LQ concentrations，70% Acid Scarlet GR and Reactive Brilliant Red K 2BP are decolorized in 9 h and 30 h，respectively. Decolorization performances of E. coli JM109 and anaerobic sludge pretreated with MHQ are improved. After MHQ pretreatment，in the presence of lawsone，80% Amaranth (1 mmol·L-1) can be decolorized in 5 h by E. coli JM109，while more than 75% Amaranth can be removed in 11 h by sludge. |
