| E.coli microbial electrodes for CellSense biosensor were prepared by polycarbonate membrane immobilization process, and their performance for heavy metals and toxic organic compounds acute toxicity determination were studied. The results showed that when E.coli was in logarithmic and stationary phase, the CellSense biosensor with E.coli showed good performance in heavy metal ions and organic pollutants acute toxicity analysis, when E.coli was in its decline phase, the stability and sensitivity of the CellSense biosensor was poor. The EC50 values of Hg2+, Cu2+, Zn2+, o-chlorophenol (2-CP) and p-nitrophenol (4-NP) detected by CellSense biosensor with E.coli were 0.6, 3.1, 5.8, 180 and 94 μg/mL, respectively. The immobilized E.coli electrodes could still suit for acute toxicity assessment after 2 months storage at 4℃. |
