| The effects NaH2PO4, adenosine disodium triphosphate (ATP), glucose 6-phosphate (G-6-P) and sodium β-glycerophosphate (G-P) on the growth and phosphatase activity of Skeletonema costatum and Prorocentrum donghaiense were studied. The results showed that both species could utilize both dissolved inorganic phosphate (DIP) and dissolved organic phosphorus (DOP), and DOP had more effects on the growth of two species than DIP. For S. costatum, after 8 days, the cell abundances of the four treatments (NaH2PO4, ATP, G-6-P and G-P) were 48×104, 73×104, 63×104 and 54×104 cells/mL, respectively; For P. donghaiense, after 10 days, the cell abundances of the four treatments were 8.7×104, 15.5×104, 12.4×104 and 9.5×104 cells/mL, respectively. On the first 3-4 days, the phosphatase activity of all treatments of the two species showed a decreasing trend, but different changes were observed for the different phosphorus substrate treatments in latter days. For the NaH2PO4 treatment, both the AP and AcP activity of two species increased from the fifth day onwards. For S. costatum, the AP activity of the ATP and G-6-P treatment groups showed no obvious changes and AcP activity had a slight increase from the fifth day to the eighth day, while the activity of G-P treatment had highest phosphatase activity which increased from the fifth day on. At the end of the experiment, the AP activity of the three DOP treatment groups (ATP, G-6-P and G-P) was 0.004×10-5, 0.014×10-5 and 0.029×10-5 U/cell, respectively, and the AcP activity was 0.006×10-5, 0.011×10-5 and 0.018×10-5 U/cell, respectively. For P. donghaiense, both the AP and AcP activity of the three DOP treatments had similar trends, i.e., ATPS. costatum had a much higher phosphatase activity and could absorb P from the environment much faster than P. donghaiense. |
