| 溶解氧及曝停比对单级自养脱氮系统微生物群落结构的影响 |
| 摘要点击 3417 全文点击 1723 投稿时间：2008-03-22 修订日期：2008-07-17 |
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| 中文关键词 单级自养脱氮 DO 曝停比 PCR-DGGE 微生物群落结构 |
| 英文关键词 To analyze the effect of DO and aeration/non-aeration ratio on the microbial community structure, active sludge and biofilm samples were taken from the SBBR one-step completely autotrophic nitrogen removal reactor under different DO concentrations and aer |
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| 中文摘要 |
| 为研究溶解氧及曝停比对单级自养脱氮系统微生物群落结构的影响，从不同溶解氧水平及曝停比条件下的SBBR单级自养脱氮反应器中采集活性污泥及生物膜样品，进行PCR-DGGE及条带统计分析.结果表明，经过1.5 a稳定运行，该系统内微生物群落结构与接种污泥相比已变得简单且较稳定.曝停比为2 h∶2 h的条件下，中高低3种溶解氧水平中，生物膜微生物群落丰富度值均高于活性污泥.DO在(曝气)2.0 mg/L(停曝) 0.4 mg/L时系统运行效能最佳，微生物群落丰富度值最高，生物膜和活性污泥样品中条带数分别约为14条和10条，微生物的多样性及相互协同代谢过程是维持单级自养脱氮系统具有较高运行效能的一个关键因素.此外，曝停比对单级自养脱氮系统微生物群落结构有较大影响.3 h∶5 h的较长曝停周期下，活性污泥与生物膜微生物组成接近，相似性为100%，各类细菌虽在活性污泥与生物膜中均能生存但活性较低，系统运行效能差. |
| 英文摘要 |
| To analyze the effect of DO and aeration/non-aeration ratio on the microbial community structure, active sludge and biofilm samples were taken from the SBBR one-step completely autotrophic nitrogen removal reactor under different DO concentrations and aeration/non-aeration ratios. By PCR-DGGE and bands statistical analyses, results show that through one and a half year of operation, the microbial community structures in this system has changed a lot compared with the inoculum. The bacterial richness values of biofilm are higher than active sludge in 2 h∶2 h aeration/non-aeration ratio, no matter when moderate, high and low DO concentration. When this system is operated under the most efficient condition: DO=2.0(aeration) mg/L,0.4(non-aeration) mg/L, the bacterial richness values are the highest. There are about 14 and 10 bands in the biofilm and active sludge samples respectively. The diversities of bacterium and their co-metabolisms are the key factors of maintaining a high operate efficiency. In addition, the aeration/non-aeration ratio also has the influence on the microbial community structure. Under long aeration/non-aeration ratio of 3 h∶5 h, microbial community structure of biofilm is similar with active sludge. The comparability is 100%. The activities of aerobic and anaerobic bacterium have lost much that makes low efficiency of this system although they could survive both in active sludge and biofilm. |
