| 基于分子技术的1株产毒藻藻际细菌多样性分析 |
| 摘要点击 2389 全文点击 1695 投稿时间：2008-05-21 修订日期：2008-07-11 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 塔玛亚历山大藻 藻际 16S rDNA克隆文库 细菌多样性 |
| 英文关键词 Alexandrium tamarense phycosphere 16S rDNA clone library microbial diversity |
|
| 中文摘要 |
| 采用构建16S rDNA克隆文库的方法，对实验室保存的1株产毒塔玛亚历山大藻在不同时期的藻际细菌群落多样性进行了分析.限制性片段长度多态性(restriction fragment length polymorphism，RFLP)结果表明，塔玛亚历山大藻藻际微生物的16S rDNA克隆文库中的克隆子总共可分为 34 种基因型，选取各谱型的代表克隆子测定其16S rDNA片段核苷酸序列，将所获得的序列与GenBank数据库进行BLAST比对，结果表明所有基因型分属于2个细菌类群：变形细菌门(Proteobacteria)和拟杆菌门(Bacteroidetes).在延滞期的藻培养液中，α-Proteobacteria占36.4%，β-Proteobacteria占9.1%，γ-Proteobacteria占27.3%，拟杆菌门(Bacteroidetes)占27.3%；在指数后期的培养液中，α-Proteobacteria占53.3%，β-Proteobacteria占13.3%，γ-Proteobacteria占6.7%，拟杆菌门(Bacteroidetes)占26.7%； 在稳定期的培养液中，α-Proteobacteria占47.8%，β-Proteobacteria占8.7%，γ-Proteobacteria占21.7%，δ-Proteobacteria占4.3%，拟杆菌门(Bacteroidetes)占17.4%；其中有不少克隆子与已知序列同源性低于 94%，表明塔玛亚历山大藻藻际环境中附着有新的未开发的微生物资源，这些细菌可能在微藻的生消过程中起着重要的调控作用，所以本研究结果在赤潮微生物调控中具有重要的理论意义和应用价值. |
| 英文摘要 |
| The composition of bacterial community in the Alexandrium tamarense culture was determined by analyzing the 16S rDNA clone libraries.16S rRNA gene was amplified by using the total DNA extracted from the A.tamarense culture at three growth stages as templates.34 different restriction fragment length polymorphism(RFLP) patterns of the clones were obtained from the three libraries.Clones representing each RFLP patterns were sequenced and phylogenetic analysis revealed that the Proteobacteria and the Bacteroidetes group can be the dominant component in the phycosphere microflora.Results showed that in the lag phase，α-Proteobacteria(36.4%)，γ-Proteobacteria(27.3%) and Bacteroidetes(27.3%) were the dominant group；in the late-exponential phase，the clones belonged to α-Proteobacteria(53.3%)，β-Proteobacteria(13.3%)，γ-Proteobacteria(6.7%)，and Bacteroidetes (26.7%)；and in the stationary phase，the community was comprised of α-Proteobacteria(47.8%)，β-Proteobacteria(8.7%)，γ-Proteobacteria(21.7%)，δ-Proteobacteria(4.3%) and Bacteroidetes(17.4%).The results also suggest that a part of the bacteria associated with A.tamarense are uncultured and novel species.And these bacteria may play a major role in regulating the processes of algal bloom initiation，maintenance and decline.So these results may provide us great academic and practical importance in controlling the algal blooms by microbes. |
