| 黄孢原毛平革菌固态发酵产漆酶的研究及应用 |
| 摘要点击 3204 全文点击 1654 投稿时间：2008-01-23 修订日期：2008-04-07 |
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| 中文关键词 黄孢原毛平革菌 固态发酵 漆酶 降解 五氯酚 |
| 英文关键词 Phanerochaete chrysosporium solid-state fermentation laccase degradation pentachlorophenol |
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| 中文摘要 |
| 对黄孢原毛平革菌（BKMF-1767）利用香蕉皮和玉米棒为发酵底物产胞外漆酶进行了研究.结果表明，当香蕉皮与玉米棒混合比例为1∶2、诱导剂CuSO4为0.4 mmol/L时，能获得最高漆酶酶活12.68 U/g.利用固态发酵所获得的粗漆酶液，进行了降解五氯酚的试验.在没有氧化还原介体时粗漆酶液能降解PCP，粗酶液中加入5 mmol/L氧化还原介体（ABTS）能获得更高的降解率，反应6 h分别为37.8%和97%.将粗漆酶液用(NH4)2SO4盐析纯化，用提纯后漆酶降解PCP，6 h后降解率为81.8%. |
| 英文摘要 |
| The potential of banana skin and corn cob as a support-substrate for the production of extracellular laccase by the white-rot fungus Phanerochaete chrysosporium (BKMF-1767) was investigated.The results indicate that laccase showed a maximum activity of 12.68 U/g when the proportion of banana skin and corn cob is 1∶2 and the inducer is 0.4 mmol/L CuSO4.In addition，crude laccase enzyme shows degradation activity to pentachlorophenol (PCP) without redox mediator or with the redox mediator (ABTS) at a concentration of 5 mmol/L，and the degradation rates of PCP were 37.8% and 97% respectively after 6 h.The crude laccase was purified by treatment of (NH4)2SO4，and the purified laccase could make the degradation rate of PCP to 81.8% within 6 h. |
