| 进水浓度或HRT变化时微好氧处理微生态机制研究 |
| 摘要点击 1886 全文点击 1455 投稿时间：2007-11-19 修订日期：2008-01-21 |
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| 中文关键词 微好氧 FISH-FCM PCR-DGGE Biolog 高浓度有机废水 HRT 微生态 |
| 英文关键词 micro-aerobic FISH-FCM PCR-DGGE Biolog high-strength organic wastewater HRT micro-ecology |
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| 中文摘要 |
| 考察改变进水COD浓度（11 000、 15 000、 30 000 mg/Ｌ）或水力停留时间（HRT为42 h、 25 h）对模拟高浓度有机废水微好氧连续小试处理效果的影响，并采用荧光原位杂交-流式细胞术（FISH-FCM）、聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE) 等分子生物学技术及Biolog FF微孔板法分析了不同处理阶段稳定期曝气柱污泥的微生态变化，以探讨进水COD浓度或HRT改变时微好氧处理效果变化的微生态机制.结果表明，在全部4个处理阶段，曝气柱污泥中酵母含量均保持在＞99.9％的水平.随着进水COD浓度的上升，污泥浓度由2.0 g/Ｌ上升到7.3 g/Ｌ，COD比去除速度由2.3 kg/（kg·d）下降到1.7 kg/（kg·d），曝气柱污泥真菌群落结构多样性指数由2.05上升至2.19，代谢多样性指数由4.42上升至4.45；而随着HRT下降，污泥浓度从7.3 g/Ｌ下降至6.0 g/Ｌ，COD比去除速度从1.7 kg/（kg·d）上升至2.8 kg/（kg·d），真菌群落结构多样性指数和代谢多样性指数则分别从2.19和4.45降至0.79和4.36.作为提高进水有机负荷的主要措施，提高进水COD浓度和缩短HRT对于废水处理效果和曝气柱微生态存在截然相反的影响. |
| 英文摘要 |
| A lab-scale continuous experiment was designed to investigate the effect of influent COD concentrations （11 000， 15 000， 30 000 mg/Ｌ）or hydraulic retention time (HRT 42， 25 h）on the microareobic treatment performance of simulated high-strength organic wastewater. The molecular microbiological technologies, including Fluorescent in-situ hybridization-Flow Cytometry (FISH-FCM), Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE), and Biolog - FF assay method, were used to detect the variation of microbial community in the aerated column during the four pseudo-steady-state periods. The yeast contents remained >99.9% throughout the overall experimental periods according to FISH-FCM. Increasing influent COD concentration brought on a rising MLSS (2.0_7.3 g/Ｌ) and a reduced specific COD removal rate ［2.3_1.7 kg/(kg·d)］, structural (PCR-DGGE)/metabolic (Biolog FF) diversity index values of fungal community in the aerated column had an increase of (2.05-2.19)/(4.42-4.45). Shortening HRT brought on a reduced MLSS (7.3-6.0 g/Ｌ) and a rising specific COD removal rate ［1.7-2.8 kg/(kg·d)］, structural (PCR-DGGE)/metabolic (Biolog FF) diversity index values of fungal community had a decrease of (2.19-0.79)/(4.45-4.36). Increasing influent COD concentration or shortening HRT has an absolutely adverse effect on the microaerobic treatment performance and micro-ecology in the aeration column although either of them can increase influent COD loading up to a higher level. |
