| 生活垃圾填埋操作方式对覆土中Ⅰ型甲烷氧化菌的影响研究 |
| 摘要点击 2972 全文点击 2147 投稿时间：2007-10-10 修订日期：2007-11-05 |
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| 中文关键词 城市生活垃圾填埋场覆土 Ⅰ型甲烷氧化菌 16S rDNA 变性梯度凝胶电泳 系统发育分析 |
| 英文关键词 landfill cover soil type Ⅰ methanotroph 16S rDNA denaturing gradient gel electrophoresis phylogenetic analysis |
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| 中文摘要 |
| 填埋场覆土中的甲烷氧化菌可削弱填埋场的温室气体释放，其中Ⅰ型甲烷氧化菌对环境条件的改变反应灵敏.实验采用16S rDNA特异性聚合酶链式反应（polymerase chain reaction, PCR）结合变性梯度凝胶电泳（denaturing gradient gel electrophoresis, DGGE）基因指纹技术研究不同填埋操作方式，包括高密度聚乙烯膜（high-density polyethylene liner, HDPE）隔离、渗滤液亚表面灌溉和植被种植，对城市生活垃圾填埋场表层覆土中Ⅰ型甲烷氧化菌的群落结构和多样性的影响.16S rDNA序列的系统发育分析结果表明，供试土壤中的Ⅰ型甲烷氧化菌均属于甲基杆菌属 （Methylobacter）.从香农-威纳多样性指数（Shannon-Wiener diversity index）和主成分分析的结果可以归纳得到：渗滤液亚表面灌溉和植被种植比HDPE膜隔离对表层覆土中Ⅰ型甲烷氧化菌群落结构的影响更大，它们会降低Ⅰ型甲烷氧化菌的多样性，并且推测渗滤液亚表面灌溉会抑制Methylobacter的生长.无HDPE膜隔离的土壤中，即有填埋气体侵入的土壤中，随填埋气体作用于土壤时间的延长，Ⅰ型甲烷氧化菌的群落结构会发生变化；覆土时间分别为3周、1.5 a和5 a的土壤中，Ⅰ型甲烷氧化菌的香农-威纳多样性指数在覆土1.5a时达到最大值. |
| 英文摘要 |
| Type Ⅰ methanotroph is crucial for methane oxidization and it responses fast to the changes in environment. In this study, 16S rDNA-based denaturing gradient gel electrophoresis (DGGE) gene fingerprint technology was applied to investigate the effect of operational modes, i.e. high-density polyethylene liner (HDPE) isolation or subsurface irrigation of landfill leachate and vegetation, on community structure and diversity of type Ⅰ methanotroph in soils covering municipal solid waste landfill. 16S rDNA based phylogenetic analysis reveals type Ⅰ methanotroph in all tested soils belongs to Methylobacter. According to Shannon-Wiener diversity index and principal component analysis, landfill leachate subsurface irrigation and vegetation have more impact on type Ⅰ methanotroph community structure and diversity than HDPE liner isolation does, and they reduce type Ⅰ methanotroph diversity. Leachate irrigation is supposed to inhibit the growth of Methylobacter population. Community structure of type Ⅰ methanotroph in landfill cover soil isolated by HDPE, i.e. invaded by landfill gas, shifts during long term gas interference. When cover age is 1.5 years old, Shannon-Wiener diversity index of type Ⅰ methanotroph reaches its maximum. |
