| 不同磷源及浓度对利玛原甲藻生长和产毒的影响研究 |
| 摘要点击 1572 全文点击 977 投稿时间：2007-10-17 修订日期：2007-12-17 |
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| 中文关键词 利玛原甲藻 磷源 碱性磷酸酶 腹泻性贝毒 |
| 英文关键词 Prorocentrum lima phosphorus source alkaline phosphatase diarrhetic shellfish poison |
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| 中文摘要 |
| 探讨了不同磷源条件下利玛原甲藻的生长情况，分析了碱性磷酸酶在营养盐利用方面的作用，对不同营养盐条件下腹泻性贝毒(diarrhetic shellfish poison, DSP)的合成情况进行了比较和分析.结果显示，以NaH2PO4、β-甘油磷酸钠和ATP分别作为唯一磷源时，NaH2PO4组和ATP组最大生长速率(μmax)差异不大，β-甘油磷酸钠组稍低； ATP组最大生物量(X)明显高于NaH2PO4组和β-甘油磷酸钠组.实验浓度范围内，β-甘油磷酸钠组碱性磷酸酶活性均比较低，而ATP组活性较高.NaH2PO4组因其浓度的变化而有较大的差异.浓度>2 μmol/L时，活性比较低，<2 μmol/L时，活性明显增高.β-甘油磷酸钠组大田软海绵酸(okadaic acid, OA)总含量和单位藻细胞OA含量最高，NaH2PO4组次之，ATP组最低.这些结果表明，利玛原甲藻可吸收利用无机磷NaH2PO4和有机磷β-甘油磷酸钠及ATP.其中，NaH2PO4和ATP的营养价值基本等效，而β-甘油磷酸钠的营养价值较低.ATP较NaH2PO4和β-甘油磷酸钠更有利于维持利玛原甲藻的生长.利玛原甲藻可以直接利用β-甘油磷酸钠； 而ATP则需要碱性磷酸酶水解后才能利用.利玛原甲藻毒素的合成与营养盐的形态有关，不同营养盐条件下DSP的合成不同.β-甘油磷酸钠为磷源时，DSP合成量最多.磷盐对DSP合成的影响与该磷盐条件下利玛原甲藻的生理状态有关. |
| 英文摘要 |
| To explore the nutrient properties of Prorocentrum lima and biosynthesis mechanism of diarrhetic shellfish poison (DSP), the growth and activities of alkaline phosphatase of Prorocentrum lima were observed under different phosphorus sources. DSP productions were also analyzed. The maximum growth rate (μmax) was slightly lower under β-sodium glycerophosphate than those under NaH2PO4 and ATP as phosphorus sources, respectively. The maximum biomass (X under ATP was higher than those under NaH2PO4 and β-sodium glycerophosphate as the phosphorus sources, respectively. When the concentration of NaH2PO4 was below 2 μmol/L, the activity of alkaline phosphatase increased significantly. However, the activities were much low in the all treatments when β-sodium glycerophosphate used as phosphorus source, whereas the activities increased with the concentration of ATP when ATP used as phosphorus source. The level of okadaic acid (OA) in Prorocentrum lima at the stationary phase under β-sodium glycerophosphate was higher than those under NaH2PO4 and ATP. These suggested that β-sodium glycerophosphate could be utilized directly by Prorocentrum lima with lower efficiency; ATP could induce alkaline phosphatase to produce inorganic phosphate for algae. DSP production in Prorocentrum lima were different under various phosphate sources, β-sodium glycerophosphate enhanced production of DSP. The difference in DSP production might be related with the physiological state of Prorocentrum lima. |
