| 菌株Sphingomonas sp. FL降解溴氨酸的特性研究 |
| 摘要点击 3238 全文点击 2904 投稿时间：2007-09-03 修订日期：2007-11-13 |
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| 中文关键词 溴氨酸 蒽醌染料 生物降解 脱色 |
| 英文关键词 bromoamine acid anthraquinone dye biodegradation decolorization |
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| 中文摘要 |
| 分离了1株溴氨酸降解菌，其可以溴氨酸为唯一碳源进行降解并使其脱色，通过16S rRNA基因序列比较和生理生化特性分析，将其归为鞘氨醇单胞菌属.溴氨酸降解和菌株生长的最适条件为：温度30℃，pH 7.0，摇床转速100 r/min ，(NH4)2SO4作为氮源，在此条件下，溴氨酸（100 mg/L）在14 h内的脱色率可达99%.低浓度NaCl（<2%）对脱色有促进作用，而高浓度NaCl（≥2%）对脱色产生抑制.以Haldane底物抑制模型表征溴氨酸初始浓度对脱色的影响，确定当初始浓度为1 393.5 mg/L 时可取得最佳比降解速率1.4 h-1.菌株不能将溴氨酸完全矿化，至反应终点52.4%的有机碳得到去除.利用GC-MS和HPLC-MS分析代谢产物显示，溴氨酸降解的中间产物是邻苯二甲酸，终产物可能为2-氨基-3-羟基-5-溴苯磺酸或2-氨基-4-羟基-5-溴苯磺酸，邻苯二甲酸可经3,4-二羟基苯甲酸途径进一步降解而被菌体利用. |
| 英文摘要 |
| A bacterial strain that could degrade bromoamine acid (BAA) as the sole carbon source was isolated. It was identified as Sphingomonas sp. based on 16S rRNA gene sequence analysis and physio-biochemical characteristics. Under the optimal growth conditions, with temperature of 30℃, pH of 7.0, rotating rate of 100 r/min and (NH4)2SO4 as the nitrogen source, the decolorization percentage of BAA (100 mg/L ) could reach 99% within 14 h. NaCl of low concentration (<2%) facilitated the decolorization, while NaCl of higher concentration (≥ 2%) had inhibition effect. The effect of initial BAA concentration on decolorizaiton could be described by the Haldane model, and the optimal specific decolorization rate of 1.4 h-1 could be obtained when the initial concentration of BAA was 1?393.5 mg/L . The strain could not mineralize BAA completely, and 52.4% of the total organic carbon was removed at the end of the reaction. The analysis of metabolites using GC-MS and HPLC-MS showed that phthalic acid was the metabolic intermediate which could be further degraded through 3,4-dihydroxybenzoic acid route and serve as the growth substrate, and the end product was estimated to be either 2-amino-3-hydroxy-5-bromobenzenesulfonic acid or 2-amino-4-hydroxy-5-bromobenzenesulfonic acid. |
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