| 处理含氮芳烃废水SBR生物反应器细菌多样性研究 |
| 摘要点击 2709 全文点击 2187 投稿时间：2007-09-17 修订日期：2007-10-26 |
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| 中文关键词 DGGE 含氮芳烃 细菌多样性 16S rRNA 基因 |
| 英文关键词 DGGE nitrogen-containing aromatic compounds bacterial diversity 16S rRNA gene |
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| 中文摘要 |
| 为了研究处理含氮芳烃废水生物反应器细菌多样性及含氮芳烃降解的微生物学机制，并为工艺改进提供依据，采集处理含氮芳烃废水生物反应器从启动到稳定高效运行过程中不同时期污泥样品进行PCR-DGGE分析.同时，从稳定运行期反应器污泥中富集培养了90株细菌，获得36株降解菌并对其中5株降解菌进行了芳烃双加氧酶活测试.结果表明，细菌种群结构自启动至稳定运行期变化明显，Acidobacteria (SBR1, SBR7)、Actinobacteria (SBR4)和β-Proteobacteria (SBR6)等类群细菌对含氮芳烃化合物降解可能起重要作用.不同研究微生物多样性方法存在各自倾向性，仅能反映细菌种群中的不同部分.在分离细菌中，Actinobacteria类群细菌为优势类群，通过对降解菌芳烃开环双加氧酶酶活分析，进一步了解了含氮芳烃在反应器内的降解机制.研究结果为含氮芳烃废水处理研究提供了一些有价值的参考依据，并丰富了含氮芳烃废水降解菌资源. |
| 英文摘要 |
| For understanding the mechanism of biodegradation in a SBR reactor treating nitrogen-containing aromatic wastewater, the reactor sludge samples were used for PCR-DGGE analysis. Meanwhile, 90 strains were isolated from the sludge sample after the start-up stage, 36 strains can degrade nitrogen-containing aromatic compounds. 5 strains nitrogen-containing aromatic degraders were further performed aromatic ring cleavage dioxygenase analysis. Result showed that bacterial diversity in the reactor were changed during the start-up stage, Acidobacteria (SBR1, SBR7), Actinobacteria (SBR4) and β-Proteobacteria (SBR6) may play important role in biodegradation of nitrogen-containing aromatic compounds, among the isolates. Different bacterial diversity research method all have bias, and reflected different part of the bacterial structure. Among the isolates, Actinobacteria were the majority, by analysis the ring cleavage enzyme activity, further understood how the four nitrogen-containing aromatic compound were degraded in the reactor. The results provided valuable references for studying microbiological degradation mechanism in the reactor, and enriched microbial resources of nitrogen-containing aromatic degrader. |
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