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咪唑乙烟酸降解菌的分离、鉴定及其降解特性研究
摘要点击 2232  全文点击 1058  投稿时间:2007-08-10  修订日期:2007-11-15
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中文关键词  咪唑乙烟酸  产碱菌属  生物降解
英文关键词  imazethapyr  Alcaligenes sp.  biodegradation
作者单位
丁伟 东北农业大学农学院哈尔滨 150030
黑龙江省农业科学院
哈尔滨 150001 
白鹤 东北农业大学资源与环境学院哈尔滨 150030 
程茁 东北农业大学资源与环境学院哈尔滨 150030 
曲娟娟 东北农业大学资源与环境学院哈尔滨 150030 
徐伟钧 黑龙江省农业科学院哈尔滨 150001 
中文摘要
      从生产咪唑乙烟酸化工厂排污口的污泥和长期施用咪唑乙烟酸的混合土壤中分离到1株能降解咪唑乙烟酸的细菌.该菌株在72 h内对500 mg/L的咪唑乙烟酸降解率达到90%以上.pH为5时,500 mg/L的咪唑乙烟酸72 h内可全部降解,而pH 8和pH 9条件下,72 h咪唑乙烟酸的降解率仅为50%左右,酸性条件比碱性条件更适合降解菌的生长.25℃和30℃条件下,降解菌对咪唑乙烟酸的降解效率较高.25℃,pH 5是降解菌对咪唑乙烟酸降解的最佳条件.从形态特征、生理生化特性及16S rRNA序列分析鉴定该菌株属于产碱菌属.
英文摘要
      One strain of imazethapyr degradable bacteria was isolated from the mixture of imazethapyr production factorys sludge and the soil that was contaminated with imazethapyr for a long time. The strain could degrade imazethapyr more than 90% within 72 h when it grew in 500 mg/L imazethapyr condition. When pH was 5, the bacteria could degrade all the imazethapyr of 500 mg/L in 72 h, but when pH was 8 and 9, only 50% imazethapyr was degraded which showed acid condition was more compatible than that of alkalescency for this bacteria growing. The higher degradation ratio at 25℃ and 30℃ was observed and the optimum condition for the bacteria to degrade imazethapyr was pH=5 and 25℃. Characterizing by physiological and biochemical properties as well as 16S rRNA sequence analysis, the strain is related and shared characteristics of the genus Alcaligenes sp..

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