| 废水脱氮与沼气脱硫耦联菌株的驯化和分离 |
| 摘要点击 1632 全文点击 1421 投稿时间：2007-05-16 修订日期：2007-09-20 |
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| 中文关键词 猪场废水 脱氮 沼气 脱硫 微生物 |
| 英文关键词 swine wastewater removal of nitrogen biogas removal of hydrogen sulfide bacteria |
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| 中文摘要 |
| 在不接种污泥、接种厌氧污泥和接种好氧污泥的条件下，采用鼓泡反应器研究猪场废水脱氮与沼气脱硫耦联反应器的启动及关键微生物.试验前期(第26 d前)，接种污泥反应器的脱氮脱硫率为50%～64%，而不接种污泥反应器的脱氮脱硫率只有11%～14%.到驯化结束时（第56 d），3个反应器的脱氮效率为90%左右，脱硫效率达到70%以上.结果表明,不接种污泥反应器经过一段时间驯化后也可以达到同样的脱氮脱硫效果，只是启动时间比接种污泥的反应器稍长.在反应器启动期间，于不同时段分别进行了微生物种群动态变化检测，结果显示微生物种群数量变化与3个反应器的脱氮脱硫效果变化趋势基本一致.在驯化成功的反应器中，分离筛选出氮硫去除率同时达到60%以上的菌株2株，初步鉴定为脱氮硫杆菌（Thiobacillus denitrificans）和假单胞菌属（Pseudomonas）细菌. |
| 英文摘要 |
| It was studied that during the startup of reactors the crucial bacteria coupling nitrogen removal from swine wastewater and hydrogen sulfide removal from biogas using bubbling absorption reactors without seeding sludge, with seeding anaerobic sludge and aerobic sludge, respectively. During the prophase of the experiment (before the 26th d), the removal rate of nitrogen and hydrogen sulfide were from 50% to 64% in reactors with seeding sludge, that in the reactor without seeding sludge was only 11%～14%. At the end of experiment (the 56th d after startup), the removal rates of nitrogen for three reactors were about 90%. The removal rates of hydrogen sulfide were more than 70%. The result showed that though the finishing time for startup of the reactor without seeding sludge was longer than that with seeding sludge, it could be obtained the same effect of removal of nitrogen and hydrogen sulfide as the latter after two months cultivation. In the different period of startup of the reactors, the variation of the population of bacteria was studied, which had the same trend as the removal effect of nitrogen and hydrogen sulfide. Two strains which could remove the nitrogen and hydrogen sulfide more than 60% simultaneously were isolated from the three reactors, and primarily identified as Thiobacillus denitrificans and Pseudomonas respectively, based on their morphology and physiological characters. |
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