| 太湖不同湖区真核微型浮游生物基因多样性的研究 |
| 摘要点击 2878 全文点击 2188 投稿时间：2007-04-23 修订日期：2007-06-19 |
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| 中文关键词 微食物网 真核微型浮游生物 DGGE 18S rDNA克隆文库 基因多样性 |
| 英文关键词 microbial food web eukarytic microplankton DGGE 18S rDNA cloning library genetic diversity |
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| 中文摘要 |
| 用DGGE (denatured gradient gel electrophoresis)和构建18S rDNA克隆文库2种方法对太湖不同湖区的真核微型浮游生物(0.8～20 μm)多样性及组成结构进行了研究.DGGE结果表明，不同湖区真核微型浮游生物的DGGE指纹图谱存在明显差异，其中营养水平较低的东太湖和贡湖DGGE条带数最多，分别为23和24，香农多样性指数分别为3.135和3.178，而营养水平较高的梅梁湾和五里湖最少，均为18，香农多样性指数为2.890，表明营养水平较低湖区的多样性高于营养水平较高的湖区.克隆测序结果表明太湖中真核微型浮游生物种类丰富，占优势的主要是一些鞭毛藻、异养鞭毛虫、纤毛虫和真菌，而营养水平不同的梅梁湾、湖心、东太湖中真核微型浮游生物组成明显不同.在营养水平较高的梅梁湾，28.6%的OUT(operational taxonomic unit)属于异养鞭毛虫，另外隐藻、金藻分别占22.9%和14.3%； 在湖心，金藻的比例最大，占25.7%，另外比较多的是异养鞭毛虫和隐藻，分别为20.0%和14.3%；而营养水平较低的东太湖各类纤毛虫所占比份最大，为26.8%，异养鞭毛虫较少，仅占4.9%，另外真菌含量较高，占12.2%. |
| 英文摘要 |
| The methods of DGGE and cloning/sequencing were used to study the diversity and community structures of small planktons (0.8～20 μm) in different areas of Lake Taihu. DGGE indicated that there were markly various fingerprints in different areas and the diversities were higher in areas with low trophic status than those with relatively high trophic status. There were 23, 24 DGGE bands in East Taihu and Gonghu Bay, respectively (Shannon index were 3.135 and 3.178) and 18 bands in both Meiliang Bay and Wuli Bay (Both shannon index were 2.890). The result of cloning/sequencing indicated that there was a high diversity of small planktons in Lake Taihu and most of them phototrophic flagellate, heterotrophic flagellate, ciliate and fungi. There were various community structures in the three different clone libraries. In Meilang Bay, 28.6% OTUs（operational taxonomic unit）belonged to heterotrophic flagellate, followed by Cryptophyta (22.9%) and Chrysophyta (14.3%). In Central Lake, 25.7% OTUs belonged to Chrysophyta, followed by heterotrophic flagellate (20.0%) and Cryptophyta (14.3%). In East Taihu，ciliates were the dominant group and only a few heterotrophic flagellates (40.9%) were detected. In addition, fungi were relatively abundant (12.2%) in this area. |
