| 转小鼠金属硫蛋白-Ⅰ基因聚球藻7002净化重金属废水的研究 |
| 摘要点击 2378 全文点击 1654 投稿时间：2007-04-16 修订日期：2007-07-27 |
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| 中文关键词 小鼠金属硫蛋白-Ⅰ 聚球藻 转基因 重金属 净化 |
| 英文关键词 mouse metallothionein-Ⅰ Synechococcus sp. PCC 7002 transgenic heavy metal decontamination |
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| 中文摘要 |
| 以转小鼠金属硫蛋白-Ⅰ基因(mMT-Ⅰ)聚球藻7002为对象，研究了其在含Cd2+、Pb2+和Hg2+的培养基中的生长特性及其对重金属的净化性能.结果表明，无论从生长速率还是对重金属的耐受特性来看，转mMT-Ⅰ聚球藻7002均明显优于野生藻；随着培养进程的延续和细胞浓度逐渐提高，体系中Cd2+、Pb2+和Hg2+的浓度逐渐降低，降幅最大的时间约在试验开始后1～3 d左右；经3 d培养，转mMT-Ⅰ聚球藻细胞对Cd2+、Pb2+和Hg2+的净化率分别为63.90%、65.99%和96.27%，吸附量分别为10.75、58.89和112.61 mg·g-1，而野生聚球藻的吸附量分别为3.40、27.01和1.12 mg·g-1，前者分别是后者的3.16、2.18和100.45倍；并建立了操作时间和细胞浓度对净化率的单因子模型，以及总括动力学模型方程，模型对实验结果拟合良好. |
| 英文摘要 |
| The growth characteristics and the decontamination of heavy metals in analogous heavy metals wastewater by transgenic Synechococcus sp. PCC 7002 with mouse metallothionein-Ⅰ gene were studied. The results show that transgenic Synechococcus sp. PCC 7002 not only has a higher tolerance to heavy metals, but also has a higher growth rate than wild strain. The concentration of Cd2+，Pb2+ and Hg2+ decreases with the progress of cultivation, and its maximum decreasing extent occurs at 1～3 day. After three days of cultivation, the absorption of Cd2+,Pb2+ and Hg2+ by transgenic Synechococcus sp. PCC 7002 is 10.75, 58.89 and 112.61 mg·g-1 of dried cells respectively, which is 3.16, 2.18 and 100.45 times higher than wild cells. The mono-factor and overall model developed fit the experiment data well. |
