| 苯并[a]芘对赤子爱胜蚓线粒体编码基因表达影响研究 |
| 摘要点击 2095 全文点击 1482 投稿时间：2007-02-27 修订日期：2007-04-02 |
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| 中文关键词 土壤污染 苯并[a]芘 赤子爱胜蚓 线粒体编码基因 抑制消减杂交 |
| 英文关键词 soil pollution benzo(a)pyrene (BaP) earthworm Eisenia fetida mitochondrion-encoded genes suppression subtractive hybridization (SSH) |
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| 中文摘要 |
| 根据ISO 11268-1:1993方法，完成了土壤中苯并［a］芘对赤子爱胜蚓（Eisenia fetida）的人工污染模拟实验.并采用Clontech PCR-SelectTM cDNA Subtration Kit构建了污染组和对照组蚯蚓之间的抑制消减文库.经测序和比对分析，在苯并［a］芘诱导上调文库中发现5个线粒体DNA（mtDNA）编码的基因，且在上调文库中具有较高的频率.它们分别为：细胞色素c氧化酶亚基Ⅰ、Ⅱ、Ⅲ，NADH氢脱氢酶亚基1，ATP合成酶亚基.研究表明，土壤中苯并［a］芘污染改变了蚯蚓线粒体基因组的表达水平，线粒体基因组表达异常是多环芳烃污染胁迫的重要分子生物标记物. |
| 英文摘要 |
| The earthworm Eisenia fetida's benzo［a］pyrene (BaP) exposure experiments were carried out in artificial soil according to ISO 11268-1:1993. And then the upregulated and downregulated subtractive cDNA libraries were constructed by Clontech PCR-SelectTM cDNA Subtration Kit. From the BaP exposure upregulated subtractive cDNA library, several cDNA segments matched mitochondrion-encoded genes were found, including cytochrome c oxidase subunitⅠ(COⅠ)，subunit Ⅱ(COⅡ), subunit Ⅲ (COⅢ)，NADH dehydrogenase subunit 1（NDH1）, and ATP synthase subunit 6. The result indicated BaP and the subsequent oxidative stress disturbed the expression of mitochondrion-encoded genes, and this was potential biomarker for oxidative stress following xenobiotic exposure. |
