| Field detection of general toxicity is urgent demand in these years, therefore rapid, sensitive, convenient biosensor was studied to detect cute toxicity of water pollutant, which is a novel optical fiber sensor immobilized bioluminent bacterial, Photobacterium phosphorem T3. This bioluminence light was measured by light detection system after it coupling to optical fiber. For the activity and bioluminence of bacterial was seriously affected by immobilizing conditions, optimization of immobilization was studied, including the concentration of Sodium alga gel and CaCl2, immobilizing time, pH, and the preservation time. When the bacteria was immobilized with 3% sodium alga in 3% CaCl2 solution for 15 minutes, the bacteria had the best activity, and the immobilized bacteria would maintain its activity for the longest time in the 3％ NaCl solution. The dose-response curves of Zn2+, NH3, nitrobenzenne and cresol are detected, and the EC50 are also calculated, which are 5.1, 10.2, 70.4, 77.0 mg·L-1. The EC50 are quite coherent to the results of standard bioluminescent bacteria method. The optical fiber biosensor could be disposable, small sized, convenient operation for field application. |
