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炭膜曝气生物膜反应器硝化作用及其微生物群落结构分析
摘要点击 1765  全文点击 994  投稿时间:2006-11-09  修订日期:2007-01-08
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中文关键词  炭膜曝气生物膜反应器  氧的利用  氨氮去除速率  荧光原位杂交(FISH)  群落结构分析
英文关键词  carbon membrane-aerated biofilm reactor (CMABR)  oxygen utilization  removal rate of NH+4-N  fluorescent in situ hybridization (FISH)  community analysis
作者单位
刘惠军 大连理工大学环境与生命学院大连116024 
杨凤林 大连理工大学环境与生命学院大连116024 
张捍民 大连理工大学环境与生命学院大连116024 
胡绍伟 大连理工大学环境与生命学院大连116024 
孙翠 大连理工大学环境与生命学院大连116024 
中文摘要
      采用炭膜曝气生物膜反应器处理无机含氮废水,通过改变进水氨氮浓度和水力停留时间,研究了反应器硝化性能、氧利用情况以及氨氮去除负荷,并对生物膜表面特性和硝化菌优势菌种进行分析.结果表明, 在膜内气压0.017 MPa,进水NH+4-N 50 mg/L,HRT为8 h条件下,NH+4-N去除率达到96%,出水NO-2-N平均为17 mg/L,一定程度上实现了短程硝化,炭膜所供给氧气被生物膜全部消耗;系统比表面氨氮最大去除速率为9.7 g/(m2·d),炭膜表面有限的生物量制约了去除速率的进一步提高; 荧光原位杂交技术分析揭示生物膜内亚硝化单胞菌(Nitrosomonas)和亚硝化螺菌(Nitrosospira)为亚硝化细菌优势菌种,分别占细菌总菌数的19%和21%,硝化螺菌(Nitrospira)为硝化细菌优势菌种,占总菌数的20%,未检测到硝化杆菌(Nitrobacter)的存在.
英文摘要
      A carbon membrane-aerated biofilm reactor was developed to treat nitrogenous inorganic wastewater. Influent NH+4-N concentrations and HRT were changed to investigate nitrification performance of reactor,oxygen utilization and NH+4-N's removal loading. Biofilm's surface characteristics and dominant bacteria of nitrifier were analyzed. The results show that under the conditions of intra-membrane pressure of 0.017 MPa, influent NH+4-N of 50 mg/L and HRT of 8 h NH+4-N removal efficiency reaches 96% and effluent average nitrite is 17 mg/L, which benefits short-cut nitrification to a certain extent. The bacteria within biofilm consume all oxygen supplied through carbon membrane. The maximum specific removal rate of NH+4-N is 9.7 g/(m2·d), which is limited by the amount of bacteria grown onto carbon membrane's surface. Fluorescent in situ hybridization analysis indicates that within the biofilm Nitrosomonas and Nitrosospira are main ammonia-oxidizing bacteria and occupy about 19% and 21% of the total bacteria number, respectively. The Nitrobacter are not observed and Nitrospira are dominant nitrite-oxidizing bacteria, the fraction of which is 20 % of total bacteria.

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