| 嗜热溶胞菌SY-14的基本特性研究 |
| 摘要点击 1399 全文点击 1062 投稿时间：2006-11-15 修订日期：2007-01-15 |
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| 中文关键词 嗜热菌 溶胞 胞外酶 土芽孢杆菌SY-14 污泥处理 |
| 英文关键词 thermophilic bacteria lysis extracellular enzyme Geobacillus sp. SY-14 sludge treatment |
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| 中文摘要 |
| 对从污泥堆肥中分离得到的具有溶胞能力的嗜热产芽孢杆菌SY-14进行了初步鉴定，并对其生长、产酶及溶胞特性进行了研究.根据菌株形态及16S rDNA测序结果，SY-14为土芽胞杆菌属(Geobacillus sp.).SY-14最适生长温度60℃左右，最适生长pH为6.0～7.0.SY-14培养上清液对完整和加热灭活后的微生物细胞都具有溶胞能力，6 h的细胞溶解率分别可达到70%和85%，上清液经过热处理后溶胞能力明显下降，表明溶胞能力主要来自于酶的作用.间歇培养过程中，SY-14培养上清液溶胞活性在对数生长期快速升高，进入稳定期前后达到最大，之后快速下降.SY-14培养上清液对受试的5株革兰氏阴性菌及部分革兰氏阳性菌都具有溶胞能力. |
| 英文摘要 |
| One spore-forming thermophilic bacterial strain SY-14, isolated from sewage sludge compost, showed significant capability to lyse bacterial cells. The strain was identified as Geobacillus sp. based on morphological characteristics and homology identification of 16S rDNA sequence. The optimal temperature and pH for growth were about 60℃ and pH 6.0～7.0 respectively. The culture supernatant of SY-14 showed lytic activity against both intact and thermal inactivated bacterial cells, and the cell lysis percentages at 6 hours were 70% and 85% respectively. The lytic activity of the culture supernatant decreased significantly after heat treatment, which inferred the lytic activity mainly derived from extracellular lytic enzymes of SY-14. The lytic activity of the culture supernatants of SY-14 increased significantly during the log phase in the batch culture process, and then decreased quickly after the maximum activity was reached. The culture supernatant of SY-14 showed lytic activity against all the five tested Gram-negative strains and some tested Gram-positive strains. |
