| Conversion of artificial corn stalk wastewater, which was prepared according to the main composition of the corn stalk fermentation lixivium, into bio-hydrogen gas by mixed culture was performed in a 20L half-continuous flow fermenter. The influences of several environmental factors on the bio-hydrogen production, such as HRT, C/N ratio, Fe2+ and artificial corn stalk wastewater concentration were discussed in the tests. The experimental results showed that HRT, C/N ratio，Fe2+ and artificial corn stalk wastewater concentration significantly affected the fermentation hydrogen production. The maximum H2 yield of 11.80mol/kg, H2 concentration of 56％and hydrogen production rate of 8.81L /(L?d) were obtained at HRT＝10h，C/N＝100，Fe2+concentration of 100mg/L and substrate concentration of 12.5g/L by mixed culture, respectively. In the fermentation hydrogen-producing process, the conversion efficiency of the substrate was more than 90%, and 39.40% of COD was removed from the reactor, and main by-products in the liquid phase were acetic acid, butyric acid , propionic acid and little ethanol and butanol throughout this study. |
