| 强抗镉蜡状芽孢杆菌的分离鉴定及其抗性机理 |
| 摘要点击 2262 全文点击 1633 投稿时间：2006-02-21 修订日期：2006-07-12 |
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| 中文关键词 镉 抗性质粒 蜡状芽孢杆菌 |
| 英文关键词 cadmium resistant plasmid Bacillus cereus |
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| 中文摘要 |
| 从北京市远郊东三岔地区废弃的铅锌尾矿中筛选到1株强抗镉的细菌.通过细菌形态学观察、扫描电镜、X射线衍射能谱分析、甲基乙酰甲醇试验与酪氨酸水解试验等特征生理生化试验、菌株“G＋C mol%”值以及16S rDNA扩增与序列测定，鉴定此菌株为蜡状芽孢杆菌（Bacillus cereus）.分离菌株可以在镉浓度为1?000 mg/L的固体培养基平板上生长，表明菌株具有强抗镉的能力.进一步分析了菌株在添加Cd2+与未添加Cd2+的液体培养基中的生长曲线，并通过质粒消除试验证明该菌株的抗镉性质与抗性质粒的存在有关. |
| 英文摘要 |
| A Gram-positive, aerobic, motile, spore-forming bacterium was isolated from a Pb-Zn mine tailing at suburb of Beijing City. The bacterial strain isolated could resist cadmium efficiently and it was able to grow well on media plates containing 1?000 mg/L cadmium. The DNA G + C content were found to be 60%. Phylogenetic analysis of the 16S rRNA gene revealed that this isolate was a member of the genus Bacillus. Two growth curves about this strain were drawn on media with and without cadmium. This strain harbors a plasmid putatively bearing essential cadmium resistant genes as demonstrated by a plasmid elimination experiment. The results of this study indicate that this strain has a better potential for cadmium resistance compared to other reports. The physiological characterization of the isolates also indicates the possible application of this strain for bioremediation of sites contaminated with cadmium. |
