| 实时定量RT-PCR方法评价壬基酚的雌激素效应 |
| 摘要点击 2532 全文点击 1448 投稿时间：2005-09-21 修订日期：2005-10-14 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 实时定量RT-PCR 壬基酚 卵黄蛋白原 基因表达 |
| 英文关键词 quantitative real-time RT-PCR nonylphenol vitellogenin gene expression |
|
| 中文摘要 |
| 壬基酚是1种环境雌激素物质,以血清卵黄蛋白原作为生物标记物检测壬基酚的最低雌激素效应浓度高于10μg/L.利用亚成体青鱼进行不同浓度的(1、10、50、100μg/L)壬基酚暴露实验,运用实时定量RT-PCR方法,从分子水平上,对青鱼VTG-Ⅰ、VTG-Ⅱ、CHG-H和CHG-L的基因表达进行了研究,并对低浓度壬基酚的环境雌激素效应进行了分析.结果表明:1μg/L浓度的壬基酚暴露组青鱼肝脏的VTG-Ⅰ、VTG-Ⅱ、CHG-H、CHG-L基因表达均被显著诱导,说明实时定量RT-PCR能够检测1μg/L壬基酚 |
| 英文摘要 |
| It has been demonstrated that nonylphenol(NP) exerts estrogenic activity.Quantitative real-time reverse transcription-polymerase chain reaction(RTPCR) was used to study the VTG-Ⅰ,VTG-Ⅱ,CHG-H and CHG-L genes expression in the liver of juvenile medaka exposed to NP at 1,10,50,100 μg/L for 60 days.The results show that the VTG-Ⅰ,VTG-Ⅱ,CHG-H and CHG-L genes expression in the liver of juvenile medaka are induced even at 1 μg/L significantly.It should be noted that the lowest-observed-effect concentration(LOECs) based on the hepatic vitellogenin(VTG) induction is about 1 μg/L,suggesting that quantitative real-time RT-PCR can detect the estrogenic activity of NP at relatively low concentration,and there is a potential application in evaluating the estrogenic activity of NP in aquatic environment. |
|
|
|
