| 栅藻对金属离子的异质敏感性与其细胞周期的关系 |
| 摘要点击 1672 全文点击 958 投稿时间：2005-06-16 修订日期：2005-07-19 |
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| 中文关键词 栅藻 异质敏感性 酯酶活性 细胞周期 流式细胞技术 |
| 英文关键词 Scenedesmus obliquus heterogeneous sensitivity esterase activity cell cycle flow cytometry |
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| 中文摘要 |
| 研究同一种群中不同状态的藻细胞对毒物的应答对理解生物体敏感性以及毒物的致毒机理十分重要.应用流式细胞技术研究了栅藻对重金属元素(Cd2+和Ni2+)的敏感性.将同一种群的栅藻细胞按其体积大小(FSC参数)分为不同组分,在暴露于毒物后分析不同组分细胞的酯酶活性.结果显示随着细胞体积的增加,其对2种金属离子的抗性逐渐增强.在分析各组分细胞的DNA量时发现栅藻的细胞大小与其所处的细胞周期具有一定关系:随着体积的增大,栅藻细胞逐渐从G1期进入G2/M期,其中,G2/M期的藻细胞对金属离子具有较强的抗性.进一步表明处于不同的细胞周期可能是决定同一种群中栅藻细胞对金属离子具有不同敏感性的重要原因. |
| 英文摘要 |
| It is important to investigate the effect of contamination on the heterogeneous cells characteristically observed within single algae species,which can be helpful to understand the sensitivity of organism and the toxicity mechanism.Flow cytometry was used to assess sensitivity to metal ion(Cd2+ and Ni2+) in Scenedesmus obliquus.By determination of esterase activity in cell fractions gated by FSC(an indicator of the cell volume),it was shown that the resistance to metal ion was improved as the cell volume increased.By simultaneously examining FSC and DNA content,a relationship between the cell volume and the cell cycle stage was observed: as the cell volume increased,the S.obliquus cells changed from G1 phase to G2/M phase.The cells in G2/M phase had more resistance to metal ion.Thus,this observation indicate that the difference of cell cycle may be the important cause of heterogeneous sensitivity to metal ion in S.obliquus. |
