| 利用umu/SOS实验评价污灌土壤的遗传毒性 |
| 摘要点击 2405 全文点击 1226 投稿时间：2005-06-06 修订日期：2005-06-30 |
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| 中文关键词 umu/SOS实验 污灌土壤 遗传毒性 |
| 英文关键词 umu/SOS test wastewater irrigated soil genotoxicity |
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| 中文摘要 |
| 利用umu/SOS显色实验评价北京市郊污水灌溉土壤中遗传毒性物质的积累,得到表征样品遗传毒性大小的β-半乳糖苷酶诱导活性(IU值).以达到阳性时诱导比率R=2(试验管IU值/对照管IU值)需要的土壤重量来反映土壤样品的相对遗传毒性.R值起初随着96孔板每孔土壤样品量的增大而增大,但在达到某一定值后呈现稳定,此时各土壤样品投加量均为每孔约含10mg土壤.用剂量效应曲线中线性段的斜率作为标准,判断出遗传毒性物质为性质相同的一类污染物,根据相关资料推测主要是由于多环芳烃类污染物积累. |
| 英文摘要 |
| umu/SOS test is used to screen the accumulation of genotoxic substances in soil irrigated with wastewater in urban Beijing. The relative genotoxicity is evaluated based on the weight of soil used for extraction that could result in a positive reaction(or the induction ratio R equals 2) and the characteristics of genotoxic substances could be evaluated by the slop of linear part of the dose/effect relationship,R reaches the maximum when the amount of soil in each cell is about 10 mg.In a case study on a wastewater or reclaimed water irrigation area, the genotoxicity of soil samples could be observed and it may be attributed to the accumulation of PAHs,according to relevant information. |
