| 木质素降解菌的分离鉴定及木素过氧化物酶的纯化 |
| 摘要点击 1566 全文点击 1586 投稿时间：2005-05-30 修订日期：2005-08-25 |
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| 中文关键词 木质素 分离鉴定 木素过氧化物酶 纯化 |
| 英文关键词 lignin isolation and identification lignin peroxidase purification |
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| 中文摘要 |
| 采用苯胺蓝脱色法,从活性污泥中分离筛选得到2株具有分泌木质素降解过氧化物酶能力的细菌菌株PKE117和PKE225.根据生理生化反应结果和16SrDNA序列分析,初步确定菌株PKE117为Pseudomanas属的1个新种,PKE225为Pseudomanas thermaerum的1个新菌株.对于菌株PKE117的发酵液依次经DEAE-纤维素32、Sephadex G-75凝胶过滤纯化,木素过氧化物酶的藜芦醇氧化比活力从0.87U/mg提高到204.5U/mg,酶的纯化倍数为235.1,回收率15%. |
| 英文摘要 |
| Two strains that could use lignin as sole carbon source and excrete peroxidases were isolated from activated sludge.Both strains are belonged to Pseudomanas based on morphological,physio-biochenical characterizatics and homology identification of 16S rDNA sequence,in which strain PKE117 is identified as a new species,while the strain PKE225 is identified as Pseudomanas thermaerum.Crude enzyme in liquid fermentation of PKE117 was analyzed with DEAE-cellulose 32 ion-exchanger resin chromatography and Sephadex G-75 gel-filtration in proper order.The lignin peroxidase specific activity increases from 0.87U/mg to 204.5U/mg,purification multiple is 235.1 and callback rate is 15%. |
