| 五氯酚对牛血清白蛋白的荧光猝灭作用 |
| 摘要点击 1425 全文点击 807 投稿时间：2005-05-29 修订日期：2005-07-06 |
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| 中文关键词 五氯酚 牛血清白蛋白(BSA) 相互作用 荧光猝灭 |
| 英文关键词 pentachlorophenol bovine serum albumin(BSA) interaction fluorescence quench |
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| 中文摘要 |
| 用荧光光谱和紫外吸收光谱法研究了五氯酚对牛血清白蛋白(BSA)的荧光猝灭作用.结果表明,五氯酚可以有规律地使BSA内源荧光猝灭,其猝灭机理可认为是五氯酚与BSA形成复合物的静态猝灭;并获得了不同温度下,五氯酚与BSA作用的结合常数和热力学参数.根据所得结果可推断五氯酚与BSA的作用力为疏水作用力和静电作用力.同时由Forster非辐射能量转移理论计算得出了五氯酚与BSA结合位置的距离. |
| 英文摘要 |
| The interaction between pentachlorophenol and bovine serum albumin(BSA) were studied using fluorescence spectra and ultraviolet-visible light absorption spectra.The results show that the fluorescence of BSA was quenched regularly by the pentachlorophenol,the mechanism of the fluorescence quench was investigated.The binding constants and thermodynamic parameters of pentachlorophenol with BSA were obtained at different temperatures.The hydrophobic interaction force and electrostatic force played a main role in the binding of pentachlorophenol with BSA.The distances of binding site between pentachlorophenol and BSA were also obtained according to the theory of Forster's non-radiative energy transfer. |
