| 烷烃高效降解菌的广谱降解性能研究 |
| 摘要点击 1812 全文点击 1015 投稿时间：2005-03-10 修订日期：2005-07-30 |
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| 中文关键词 烷烃 降解菌 原油 姥鲛烷 5α-雄甾烷 六甲苯 |
| 英文关键词 alkanes bacterium stain crude oil 5α-androstane hexamethylbenzene pristane anaerobic aerobic |
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| 中文摘要 |
| 针对分离、筛选出的正烷烃高效降解菌株C-14-1的广谱降解性能进行研究.结果表明,C-14-1菌株不仅对较高浓度(大于50mg/L)的正烷烃降解能力显著,对低浓度(约2mg/L)的正烷烃系列同样具有较好的降解效果,并且降解率和烷烃的碳原子数紧密相关.经过48h的发酵,当烷烃碳原子数为10～22时,降解率基本上大于85%;当烷烃碳原子数大于22时,降解率降低到15%～78%.C-14-1菌株对浓度很低(约0.3mg/L)的原油也显示出一定的降解效果.当烷烃碳原子数为12～22时,降解率平均为65%;当烷烃碳原子数大于22时,降解率降低为平均28.3%.当试验系统处于限氧状态时,C-14-1菌株对5α-雄甾烷和六甲苯基本上无降解作用,但对姥鲛烷的降解率却高达97%.在供氧充足的通气状态下以5α-雄甾烷为唯一碳源时,C-14-1菌株对5α-雄甾烷的降解率达到77%.当与其它低碳原子数的正烷烃共存时,5α-雄甾烷更容易被C-14-1菌株通过协同代谢而降解.通气培养48h时,其降解率达到100%;即使在限氧状态下,其降解率也能达到64%. |
| 英文摘要 |
| Degradation capability of a bacterium strain C-14-1 for various n-alkanes was investigated.Experimental results indicate that C-14-1 can quite effectively degrade n-alkanes not only with a high concentration(>50mg/L individual alkane),but also with a low concentration(2mg/L individual alkane).Moreover,the degradation efficiency is closely related to the carbon number of(nalkanes.) After 48h cultivation,the degradation efficiency is generally greater than 85% for n-alkanes with a carbon number between 10 and 22;while the degradation efficiency decreases to 15%～78% if the carbon number is larger than 22.C-14-1 also exhibits certain degradation efficiency for a low-concentration crude oil(about 0.3mg/L).The average degradation efficiency of crude oil reaches 65% when the carbon number of n-alkanes is between 10 and 22;while the degradation efficiency drops to 28.3% if the carbon number is larger than 22.Under anoxic condition,C-14-1 can hardly degrade 5α-androstane and hexamethylbenzene;however,it can effectively degrade pristane,achieving a degradation efficiency of 97%.Under aerobic condition,the degradation efficiency of 5α-androstane reaches 77% if used as the sole carbon source.However,5α-androstane can be effectively degraded by(C-14-1) under either aerobic or anaerobic condition if there coexist other short-chain n-alkanes.After 48 h cultivation,all 5α-androstane can be removed under aerobic condition;while its degradation efficiency still reaches 64% even under anoxic condition. |
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