| 酸性土壤环境石油烃生物降解效应 |
| 摘要点击 1607 全文点击 1499 投稿时间：2004-08-23 修订日期：2004-10-18 |
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| 中文关键词 石油烃 生物降解 酸性土壤环境 抑制效应 生物载体 |
| 英文关键词 hydrocarbons biodegradation acid soils restraint bio-carriers |
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| 中文摘要 |
| 污染场地酸性土壤环境和生物修复土壤酸化,使得酸性土壤环境石油烃生物降解效应影响与有效调控成为污染土壤修复的重要内容.本文通过监测酸性和偏碱性土壤中微生物数量、活性以及石油烃含量变化,探讨酸性环境对除油微生物及烃降解效率的影响.通过投加富集菌液和生物载体,调控酸性土壤微生态环境,揭示微环境调控对于烃生物降解效应影响.研究结果表明,pH为5.4～5.7的酸性土壤,对土著除油微生物活性和数量具有显著抑制性,烃降解处于停滞状态.投加富集菌液未能有效地减弱酸性环境对除油微生物的强烈抑制作用.微生物数量在14d内从106个/ g减至0,微生物FDA(FluoresceinDiacetate)活性很低,约0.10Abs/g .生物载体的投加,能有效改善介质界面微生态环境,明显减弱酸性环境的抑制效应,减缓除油微生物死亡速率.19d时土壤中降解微生物由原来的2×106个/g下降到2.2×102个/g ,第49d石油烃的生物降解率为13.02%. |
| 英文摘要 |
| Biodegradation efficiency of hydrocarbons and melioration of micro-ecosystem conditions in acid soils should be seriously concerned due to either occurrence of acid polluted soils or acidification during bioremediation process. The influence of acid situation on degrading microbes and the biodegradation rate were figured out by monitoring variations of biomass, microbial activities and petroleum contents with time in acid and alkaline polluted soils in laboratory. Injecting degrading microbes and meliorating micro-ecosystem conditions of acid soils were conducted. The results showed that acid soils (pH=5.4～5.7) had extreme restraint on local microbe numbers and activities, and biodegradation rate almost reached zero. Injection of degrading microbes could not remarkably reduce the restriction of acid conditions. The microbe numbers quickly went down from 106 cells/ g dried soil to zero in 14 days and the Fluorescein Diacetate(FDA) activities were only about 0.10Abs/g dried soil. However, addition of bio-carriers could effectively improve micro-ecosystem conditions in acid soils, thus notably diminish the restraint to some extent. In 19 days, the amount of microorganisms decreased from 2×106 to 2.2×102cells/g dried soil. And up to 49 days, about 13% hydrocarbons were removed from the polluted soils by microbes. |
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