| 微囊藻毒素-LR完全抗原的设计及制备 |
| 摘要点击 1360 全文点击 1166 投稿时间：2004-09-06 修订日期：2004-10-15 |
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| 中文关键词 微囊藻毒素-LR 完全抗原 偶联比 |
| 英文关键词 Microcystin-LR complete antigen coupling ratio |
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| 中文摘要 |
| 从偶联位点、偶联剂、载体蛋白和偶联步骤等分析出发,设计了微囊藻毒素-LR (Microcystin-LR ,MC-LR)完全抗原的制备方法.在半抗原分子第7位氨基酸分子上引进1个自由的氨基;再用戊二醛2步法将此中间产物(H2N-etMC-LR)分别与BSA和OVA偶联.中间产物和偶联产物分别经固相萃取和透析纯化后,经SDS凝胶电泳、紫外扫描及生物质谱技术鉴定,结果表明MC-LR与BSA的平均偶联比能达到5以上,满足了进一步免疫的要求. |
| 英文摘要 |
| Based on the analysis of coupling location, coupling regent, carrying protein and coupling process, completed antigen of Microcystin-LR (MC-LR) was synthesized. A free amidogen was introduced to microcystin-LR (MC-LR) using chemical modification (aminoethylation) of its 7th core amino acids, N-methyl-dehydroalanine. The intermediate product Aminoethyl-MC-LR (H2N-etMC-LR) was purified by Solid Phase Elute (SPE) and identified by Mass Spectrometry,then coupled with BSA through glutaraldehyde to be complete antigen. The completed antigen was dialyzed and identified by SDS-PAGE,UV-scanning and bio-mass spectrometry. The result show that the coupling ratio between MC-LR and BSA of complete antigen in average was over 5, and it is sufficient to immunity. |
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