| 2株海洋石油降解细菌的降解能力 |
| 摘要点击 2587 全文点击 2642 投稿时间：2003-10-31 修订日期：2004-01-13 |
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| 中文关键词 海洋石油污染 生物降解 假单胞菌 16SrDNA 双加氧酶基因 |
| 英文关键词 marine petroleum contamination biodegradation Pseudomonas 16S rDNA,dioxygenase gene |
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| 中文摘要 |
| 为实施海洋石油污染的生物治理,从厦门储油码头油污水中分离得到了2株石油降解菌.它们能够在以柴油、萘或芘为唯一碳源的培养基中生长,并适应于海洋环境的温度、pH和盐度.它们对萘都有很强的降解能力,3d内降解率可达87.53%和84.01%;7 d内对芘降解率分别为8.35%、5.37%.经16S rDNA同源性分析表明2菌株之间及其与施氏假单胞菌的同源性皆为99%;Biolog生化鉴定也表明它们为不同的菌株.通过兼并PCR扩增,序列分析发现2种菌编码完全相同的萘双加氧酶基因,该基因与其它假单胞菌的萘双加氧酶大亚基有98%同源性. |
| 英文摘要 |
| Two bacterial strains, able to degrade both diesel fuel and PAHs, were isolated from the oil sewage of oil storage dock in Xiamen. Analysis of 16S rDNA sequences showed that both strains had high homology (99%) with Pseudomonas stutzeri and should be classified to this species. But Biolog analysis showed they were quite different strains. Tests of temperature,salinity,pH on cell growth suggested that they were from marine environment. Both showed high degradation activity to naphthalene,87.53% and 84.01% within 3 days;and slight activity to pyrene,8.35% and 5.37% within 7 days. And both harbored naphthalene dioxygenase genes of 98% identity with other those from Pseudomonas strains. |
