| 蛋白酶对水环境中病毒的灭活作用 |
| 摘要点击 1256 全文点击 1450 投稿时间：2003-10-31 修订日期：2003-12-23 |
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| 中文关键词 蛋白酶 病毒 噬菌体T4 灭活 消毒 |
| 英文关键词 proteinase virus inactivation T4 phage disinfection |
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| 中文摘要 |
| 以大肠杆菌噬菌体T4作为模式病毒,研究了蛋白酶对病毒的灭活作用.试验结果表明:蛋白酶灭活病毒的效果明显. 适宜条件下,67.5u/mL的蛋白酶K处理1h对纯水中病毒(6.2×105PFU/L)和生活污水中病毒(7.2×105PFU/L)灭活率分别达到了99.4%和49.4%,处理3h的灭活率分别是>99.9%和81.1%;工业蛋白酶1398在75.0u/mL酶浓度下处理1h对纯水水中病毒(1.7×105 PFU/L)灭活率达到74.4%.pH和温度对病毒灭活效果的影响不明显. |
| 英文摘要 |
| The inactivation effectiveness of proteinase to viruses was investigated by using T4 phage as a model virus. The results showed that the inactivation effectiveness of proteinase to T4 phage was obvious. In the optimum conditions and 67.5u/mL concentration, the inactivation rate of proteinase K to T4 phage in sterilized water and in sewage achieved 99.4% and 49.4% respectively in an hour, and achieved >99.9% and 81.1% in three hours. The inactivation rate of the industrial proteinase 1398 to T4 phage in sterilized water achieved 74.4% in an hour. The effects of pH and temperature on the inactivation effectiveness was not evident. |
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