| 甲基汞对大鼠脑即刻早期基因c-jun mRNA表达影响 |
| 摘要点击 2090 全文点击 1934 投稿时间：2003-10-16 修订日期：2003-12-25 |
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| 中文关键词 甲基汞 c-jun 基因表达 RT-PCR 早期预报 |
| 英文关键词 methyl mercury chloride immediate early gene c-jun gene expression RT-PCR early prediction |
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| 中文摘要 |
| 为了探讨甲基汞神经毒性早期预报指标和信息传递分子机制,本文应用RT-PCR方法研究了不同浓度甲基汞暴露不同时间后,大鼠脑中即刻早期基因c-jun mRNA表达变化(对照组为0.9%生理盐水、暴露组浓度分别为0.05、0.5、5 mg·kg-1;取样时间分别为20、60、240、1440 min).结果表明大鼠脑c-jun mRNA表达优先于汞的蓄积,大鼠脑c-jun mRNA表达变化对于甲基汞神经毒性具有早期预报作用, 即刻早期基因c-jun参与了甲基汞对中枢神经系统损害的毒性过程. |
| 英文摘要 |
| In order to probe into the early prediction molecular index and the signal transduction molecular mechanism of methyl mercury chloride (MMC) neurotoxicity, the expression of c-jun mRNA in rat brains induced by different concentration MMC for different times were observed by using reverse transcription polymerase chain reaction (RT-PCR) methods (the control group was physiological saline of 0.9%, the concentrations of expose groups were 0 05,0.5,5 mg·kg-1 respectively ,the sampling times were 20,60,240,1440min).The result showed the expression of c-jun mRNA in rat brains was prior to the accumulation of mercury , and the expression of c-jun mRNA in rat brains could early predict the neurotoxicity of MMC. IEG( c-jun) participated in the toxicity process of injury by MMC. |
