| 乙醛对脱氧核糖核酸分子的损伤作用 |
| 摘要点击 1863 全文点击 2424 投稿时间：2003-08-21 修订日期：2003-11-14 |
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| 中文关键词 乙醛 核酸损伤 DNA链断裂 DNA-DNA交联 DNA-蛋白质交联 8-羟基脱氧鸟苷 |
| 英文关键词 acetaldehyde DNA damage DNA strand breakage DNA-DNA crosslinks DNA-protein crosslinks 8-Hydroxy-2′- deoxyguanosine(8-OHdG) |
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| 中文摘要 |
| 应用单细胞凝胶电泳技术和液相色谱-电化学检测法研究了典型环境污染物乙醛对脱氧核糖核酸(DNA)分子的损伤效应.结果表明:乙醛不仅可诱导人外周血淋巴细胞DNA发生链断裂,还可引起DNA-DNA,DNA-蛋白质交联;乙醛与小牛胸腺DNA的体外作用较弱,但在铁离子介导下对DNA的氧化能力增强,可产生一定量的8-羟基脱氧鸟苷(8-OHdG)加合物;动物实验表明,乙醛诱导大鼠肺组织DNA氧化损伤生成8-OHdG, 提示乙醛具有潜在遗传毒性,可能与引起DNA交联及氧化有关. |
| 英文摘要 |
| The effects of DNA damage induced by the typical environmental pollutant acetaldehyde were studied with single cell gel electrophoresis(SCGE) and high performance liquid chromatography with electrochemical detection(HPLC-EC). The results showed that acetaldehyde not only could cause DNA strand breakage but also DNA-DNA, DNA-protein crosslinks of lymphocytes of human peripheral blood. The reaction of acetaldehyde with DNA in vitro was weak, but the oxidative ability was enhanced and the reaction could produce a number of 8-OHdG adducts mediated by the Fe2+. The animal experiment shows that acetaldehyde can cause the oxidative DNA damage of rat lung tissues, which suggests that acetaldehyde have the potential genotoxicity and its chemical mechanism is relative to the crosslinks and oxidation with DNA. |
